AM20365PU-N Smallpox B5R (33-47) antibody

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Mouse anti Smallpox B5R 1J2F3

Product Description for Smallpox B5R

Mouse anti Smallpox B5R 1J2F3.
Properties: (33-47)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Smallpox B5R

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Cowpox virus, Ectromelia virus, Monkeypox virus, Rabbitpox virus, Vaccinia virus
Applications WB
Clonality Monoclonal
Clone 1J2F3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Amino acids 33-47 of the B5R protein.
AA Sequence:
The amino acid sequence used as immunogen is 100% homologous in Variola virus, 93% homologus in Cowpox virus, Camelpox virus and Taderapox virus, 86% homologus in Vaccinia virus and 80% homologus in Ectromelia virus, Rabbitpox virus and Monkeypox virus.
Property 33-47
Isotype control AM03095PU-N
Application Western blot: 1-3 µg/ml.
Recommended Positive Control: A partial recombinant fusion protein was used to test.
Background B5R, a 561 amino acid membrane protein containing ankyrin repeats that mediate protein-protein interactions and is related to the regulators of complement activation (RCA) superfamily. It consists of four short consensus repeats (SCRs) and transmembrane anchor region or the CT (cytoplasmic tail). Its transmembrane domain is the major determinant for targeting the B5R protein to the outer membrane of EEV (extracellular enveloped virus) and for supporting EEV formation, thus playing an important role in the process of virus envelopment and release. It may also be involved in viral evasion from host immune responses. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.
Concentration 0.5 mg/ml
General Readings
  1. Massung,R.F., Loparev,V.N., Knight,J.C., Totmenin,A.V., Chizhikov,V.E., Parsons,J.M., Safronov,P.F., Gutorov,V.V., Shchelkunov,S.N. and Esposito,J.J. Terminal region sequence variations in variola virus DNA. Virology 221 (2), 291-300 (1996).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography
Buffer System:
PBS containing 0.2% Gelatin and 0.05% Sodium Azide
Liquid purified Ig fraction
Species Reactivity
Species reactivity (expected):
Cowpox virus, Ectromelia virus, Monkeypox virus, Rabbitpox virus, Vaccinia virus
Species reactivity (tested):
Variola virus.
Recognizes Smallpox B5R.

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