AP31966PU-N Smgc antibody

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0.1 mg / €340.00
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Quick Overview

Goat anti Mouse Smgc

Product Description for Smgc

Goat anti Mouse Smgc.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for Smgc

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Muc19, Submandibular gland protein C
Presentation Aff - Purified
Reactivity Ms
Applications E, ICC/IF
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6JHY2
Immunogen:
Synthetic peptide: KLEPKYENPTNGS with a Cysteine residue linker, corresponding to internal sequence amino acids 518-530 of Mouse Muc19 (NP_945121.1).
AA Sequence:
KLEPKYENPTNGS
GeneID:
223809
Application
Peptide ELISA: 1/16000 (Detection limit). 
Western blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Immunocytochemistry: 4 µg/ml. Positive staining of select epithelial cells in the murine Submanibular gland at E18. Data provided by Melinda Larsen, State University of New York, Albany, NY.
Background Detected in terminal tubule cells of the submandibular gland (at protein level). Expressed in submandibular salivary glands of 3-day-old males but not adults. Expression in adult submandibular glands is restricted to females. Isoform 5 is expressed in both 3-day-old and adult sublingual glands.
Concentration 0.5 mg/ml
Product Citations
Purchased from Acris:
  1. Yamamoto M, Nakata H, Kumchantuek T, Sakulsak N, Iseki S. Immunohistochemical localization of keratin 5 in the submandibular gland in adult and postnatal developing mice. Histochem Cell Biol. 2016 Mar;145(3):327-39. doi: 10.1007/s00418-015-1392-5. Epub 2015 Dec 15. PubMed PMID: 26671786.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody recognizes Mouse Muc19 /Smgc.
Other species not tested.

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