NSB1085 Smooth Muscle Myosin Light Chain Kinase antibody

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Rabbit anti Bovine, Mouse, Rabbit Smooth Muscle Myosin Light Chain Kinase

Product Description for Smooth Muscle Myosin Light Chain Kinase

Rabbit anti Bovine, Mouse, Rabbit Smooth Muscle Myosin Light Chain Kinase.
Product is tested for Western blot / Immunoblot.

Properties for Smooth Muscle Myosin Light Chain Kinase

Product Category Primary Antibodies
Quantity 0.1 ml
Reactivity Bov, Ms, Rb
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760. The sequence is conserved in human, mouse and rat.
Add. information Research Areas: Cancer
Application Western Blot, This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. , Western Blot
Background Smooth Muscle Myosin Light Chain Kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction (SMC), and a key element in ligand-mediated endothelial cell gap formation and vascular permeability, motility and morphology. Smooth muscle MLCK exists in at least two isoforms, short (~150 kDa) and long (210 kDa) which are identical except for an extended amino terminus with two additional putative actin-binding motifs in the long isoform. MLCK is phosphorylated by several kinases including protein kinase A (PKA), and mediates its function by phosphorylating 20 kDa myosin light chain (MLC20). Phosphorylation of MLCK inhibits the actin-activated ATPase of myosin II by reducing its affinity for actin. MLCK possesses a counter-balancing role in vascular regulation, by mediating vasoconstriction via direct action on SMCs and vasodilation via action on endothelial cells (ECs).
Concentration 1 mg/ml
General Readings Isotani, E., et al. (2004) Real-time evaluation of myosin light chain kinase activation in smooth muscle tissues from a transgenic calmodulin-biosensor mouse. Proc. Nat'l. Acad. Sci. USA 101(16):6279-6284.
Dulyaninova, N.G. and A.R. Bresnick (2004) The long myosin light chain kinase is differentially phosphorylated during interphase and mitosis. Exp. Cell Res. 299(2):303-314.
Totsukawa, G., et al. (2004) Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts. J. Cell. Biol. 164(3):427-439.
Tinsley, J.H., et al. (2004) Isoform-specific knockout of endothelial myosin light chain kinase: closing the gap on inflammatory lung disease. Trends Pharmacol. Sci. 25(2):64-66.
Kamm, K.E. and J.T. Stull (2001) Dedicated myosin light chain kinases with diverse cellular functions. J. Biol. Chem. 276(7):4527-4530.
Takaishi, K., et al. (2000) Localization and activity of myosin light chain kinase isoforms during the cell cycle. J. Cell. Biol. 151(3):697-708.
Watanabe, H., et al. (2001) Myosin light-chain kinase regulates endothelial calcium entry and endothelium-dependent vasodilation. FASEB J. 15(2):282-284.
Smith, L., et al. (2002) Properties of long myosin light chain kinase binding to F-actin in vitro and in vivo. J. Biol. Chem. 277(38):35597-35604.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Smooth Muscle Myosin Light Chain Kinase [Ser1760]
Bovine, Mouse, Rabbit
Gene ID 213435

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