BM5081 Smoothelin antibody

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0.1 ml / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Canine, Chicken, Feline, Human, Monkey, Porcine, Rat Smoothelin R4A

BM5081

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  • BM5081

Product Description for Smoothelin

Mouse anti Canine, Chicken, Feline, Human, Monkey, Porcine, Rat Smoothelin R4A.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Smoothelin

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms SMSMO, SMTN
Presentation Purified
Reactivity Can, Chk, Fe, Hu, Mky, Por, Rt
Applications C, E, ICC/IF, P, WB
Clonality Monoclonal
Clone R4A
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P53814
Immunogen:
Cytoskeletal extract of Chicken gizzard
GeneID:
6525
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Add. information Smoothelin was not detetced in primary or long term smooth muscle cell cultures.
Application Western Blot: 1/1000.
Cytological Material.
Immunofluorescence Microscopy. 
Immunocytochemistry.
Immunohistochemistry on Frozen and Paraffin Sections:
 1/100-1/200 with PBS, pH 7.4 (ABC Method). 
Microwave pretreatment is required.
Incubation Time: 1 h at RT, extended with Paraffin.
Background Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
Concentration 1.0 mg/ml
General Readings
  1. van der Loop FT, Schaart G, Timmer ED, Ramaekers FC, van Eys GJ. Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells. J Cell Biol. 1996 Jul;134(2):401-11. PubMed PMID: 8707825. (Free PMC Article available)
  2. Wehrens XH, Mies B, Gimona M, Ramaekers FC, Van Eys GJ, Small JV. Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform. FEBS Lett. 1997 Apr 1;405(3):315-20. PubMed PMID: 9108311.
  3. van der Loop FT, Gabbiani G, Kohnen G, Ramaekers FC, van Eys GJ. Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype. Arterioscler Thromb Vasc Biol. 1997 Apr;17(4):665-71. PubMed PMID: 9108778.
  4. Christen T, Bochaton-Piallat ML, Neuville P, Rensen S, Redard M, van Eys G, et al. Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation. Circ Res. 1999 Jul 9;85(1):99-107. PubMed PMID: 10400915.
  5. Johansson B, Eriksson A, Ramaekers F, Thornell L. Smoothelin in adult and developing human arteries and myocardium. Histochem Cell Biol. 1999 Oct;112(4):291-9. PubMed PMID: 10550614.
  6. Johansson B, Eriksson A, Ramaekers F, Thornell LE. Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts. Histochem J. 1999 Nov;31(11):723-7. PubMed PMID: 10646837.
  7. Deruiter MC, Rensen SS, Coolen GP, Hierck BP, Bergwerff M, Debie WM, et al. Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform. Dev Dyn. 2001 Aug;221(4):460-3. PubMed PMID: 11500983.
  8. Council L, Hameed O. Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma. Mod Pathol. 2009 May;22(5):639-50. doi: 10.1038/modpathol.2009.9. Epub 2009 Feb 27. PubMed PMID: 19252475.
  9. Coco DP, Hirsch MS, Hornick JL. Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract. Am J Surg Pathol. 2009 Dec;33(12):1795-801. PubMed PMID: 19950405.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Monkey, Canine, Feline, Porcine and Chicken
Specificity
Specificity:
This antibody is specific for Smoothelin.
Reactive with a novel cytoskeletal protein specific for smooth muscle cells: smoothelin.
In Western Blot 2 Molecular weight isoforms are detected: 59 kD isoform specific to visceral SMC (Smoothelin A) and 100 kD isoform in vascular SMC (Smoothelin B).
The antibody is very useful for characterization of atherosclerotic lesions.
The antibody is useful tool to monitor SMC differentiation.
Confocal scanning laser microscopy of tissue sections and cells in culture showed a filamentous organization of smoothelin co-localizing with actin stress fibers.
Gene ID 6525

Accessory Products

Proteins and/or Positive Controls

Proteins for Smoothelin (3 products)

Catalog No. Species Pres. Purity   Source  

Smoothelin (transcript variant 2)

Smoothelin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Smoothelin

Smoothelin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Smoothelin

Smoothelin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Positive controls for Smoothelin (4 products)

Catalog No. Species Pres. Purity   Source  

Smoothelin Lysate

Western Blot: Smoothelin Lysate [NBL1-16257] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SMTN
0.1 mg / €400.00
  Novus Biologicals Inc.

SMTN overexpression lysate

SMTN overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

SMTN overexpression lysate

SMTN overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.

SMTN overexpression lysate

SMTN overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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