discontinued

GTX28969 Smoothelin antibody

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0.1 mg / €410.00

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Mouse anti Chicken, Human, Monkey Smoothelin R4A

GTX28969

Product Description for Smoothelin

Mouse anti Chicken, Human, Monkey Smoothelin R4A.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Smoothelin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Chk, Hu, Mky
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone R4A
Host Mouse
Isotype IgG1
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Immunogen
Immunogen:
Cytoskeletal extract of chicken gizzard.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ICC, IHC-Fr, IHC-P, WB
Background Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin, vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. A single copy gene encodes human smoothelin, which is located on chromosome 22. The monoclonal smoothelin antibody R4A is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
Concentration 0.5 mg/ml
Storage Keep as concentrated solution. Store at 4
Format
Purification:
Protein G affinity purified.
Buffer System:
Preservative: 0.09% Sodium Azide Constituents: PBS
Purity:
Protein G affinity purified.
State:
Liquid
Purified
Specificity
Specificity:
Cross-reacts with Human, Dog, Pig, Cat, Chicken and Monkey. Does not cross-react with Rat, Mouse or Hamster. Not yet tested in other species.

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