discontinued

ARP41213_P050 Splicing factor 1 (SF1) antibody

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Rabbit anti Canine, Human, Mouse, Rabbit Splicing factor 1 (SF1)

Product Description for Splicing factor 1 (SF1)

Rabbit anti Canine, Human, Mouse, Rabbit Splicing factor 1 (SF1).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Splicing factor 1 (SF1)

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms BBP, Mammalian branch point-binding protein, Transcription factor ZFM1, ZFM1, ZNF162, Zinc finger gene in MEN1 locus, Zinc finger protein 162
Presentation Aff - Purified
Reactivity Can, Hu, Ms, Rb
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer AVIVA Systems Biology

Datasheet Extract

Immunogen
Swiss Prot Num:
Q15637
Immunogen:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human SF1 with an internal ID of P22588
GeneID:
7536
Add. information Rabbit Ig G is purified by peptide affinity chromatography method. 69kDa
Application Suggested dilution at 1 ?g/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titre using peptide based assay: 1:12500
Background SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5\'-UACUAAC-3\' of the pre-mRNA. SF1 may act as transcription repressor.This gene encodes a member of the heat shock factor (HSF) family of transcriptional activators for heat shock proteins. This gene is a candidate gene for azoospermia, since it localizes to a region of chromosome Y that is sometimes deleted in infertile males. The genome has two identical copies of this gene within a palindromic region; this record represents the more centromeric copy. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
General Readings Rino,J., (2008) Mol. Cell. Biol. 28 (9), 3045-3057
Storage Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20DegC. Avoid repeat freeze-thaw cycles.
Format
Purification:
Rabbit Ig G is purified by peptide affinity chromatography method.
Buffer System:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20DegC. Avoid repeat freeze-thaw cycles.
State:
Aff - Purified
Specificity
Species:
Human
Gene ID 7536

Accessory Products

Proteins and/or Positive Controls

Proteins for Splicing factor 1 (SF1) (6 products)

Catalog No. Species Pres. Purity   Source  

Splicing factor 1 (SF1) (transcript variant 3)

Splicing factor 1 (SF1) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Splicing factor 1 (SF1)

Splicing factor 1 (SF1) Human
  Abnova Taiwan Corp.

Splicing factor 1 (SF1)

Splicing factor 1 (SF1) Human in vitro transl.
  Abnova Taiwan Corp.

Splicing factor 1 (SF1)

Splicing factor 1 (SF1) Human in vitro transl.
  Abnova Taiwan Corp.

Splicing factor 1 (SF1)

Splicing factor 1 (SF1) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Splicing factor 1 (SF1)

Splicing factor 1 (SF1) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Splicing factor 1 (SF1) (1 products)

Catalog No. Species Pres. Purity   Source  

SF1 Lysate

Western Blot: SF1 Lysate [NBL1-15873] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SF1
  Novus Biologicals Inc.
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