discontinued

NSB912 SRC Family Negative Regulatory Phosphorylation Site antibody

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Rabbit anti Human SRC Family Negative Regulatory Phosphorylation Site

NSB912

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  • NSB912

Product Description for SRC Family Negative Regulatory Phosphorylation Site

Rabbit anti Human SRC Family Negative Regulatory Phosphorylation Site.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for SRC Family Negative Regulatory Phosphorylation Site

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide (Human).
Application Western Blot, Western Blot Predicted molecular weight 60 kDa. , Western Blot 0.35 - 1 ug/ml
Background The Src family of non-receptor tyrosine kinases is involved in the modulation of a wide variety of normal and pathogenic cellular processes including growth, development, differentiation, and carcinogenesis. These proteins contain a consensus structure comprised of one or more N-terminalSH2 and/or SH3 domains involved in protein-protein interactions, a central catalytic tyrosine kinase domain, and a short C-terminal domain. This C-terminal domain contains a tyrosine residue that, when phosphorylated by Csk tyrosine kinase, functions as a negative regulatory site (tyrosines 530, 531, 522, 505, and 508 in Src, Fyn, Hck, Lck, and Lyn, respectively) by providing a binding site for the N-terminal SH2 domain. The association of these two domains blocks access to a critical tyrosine residue in the activation loop, the phosphorylation of which is required for enzyme activation. When the C-terminal domain tyrosine residue is dephosphorylated, the catalytic domaintyrosine can be maximally phosphorylated leading to full kinase activation.
Concentration 0.5 mg/ml
General Readings General / background references:Young MA et al. Dynamic coupling between the SH2 and SH3 domains of c-Src and Hck underlies their inactivation by C-terminal tyrosine phosphorylation. Cell 105:115-26 (2001). PubMed PMID: 11301007
Hubbard SR & Till JH Protein tyrosine kinase structure and function. Annu Rev Biochem 69:373-98 (2000). PubMed PMID: 10966463
Egan C et al. Activation of Src in human breast tumor cell lines: elevated levels of phosphotyrosine phosphatase activity that preferentially recognizes the Src carboxy terminal negative regulatory tyrosine 530. Oncogene 18:1227-37 (1999). PubMed PMID: 10022129
Thomas SM & Brugge JS Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13:513-609 (1997). PubMed PMID: 9442882
Thomas SM & Brugge JS Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13:513-609 (1997). PubMed PMID: 9442882
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
SRC Family Negative Regulatory Phosphorylation Site [Tyr522]
Species:
Human

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