discontinued

NB100-66337 SRC antibody

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0.1 mg / €430.00

Quick Overview

Human anti Human SRC 3828

Product Description for SRC

Human anti Human SRC 3828.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for SRC

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Schmidt Ruppin A 2, Schmidt Ruppin A2
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 3828
Host Human
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Recombinant human SRC
Application Western Blot, This antibody is useful for Western Blot, Western Blot 1/100 - 1/500
Background Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. The tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of tyrosine 418 at the catalytic domain, thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated and Src becomes active. Src is a proto oncogene that may play a role in the regulation of embryonic development and cell growth. Mutations in this gene could be involved in the malignant progression of colon cancer. Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Affinity purified
Buffer System:
No Preservative
Purity:
Affinity purified
State:
Aff - Purified
Specificity
Specificity:
NB100-66337 recognises the human protein tyrosine kinase Src, a 60kD protein that is the prototypic member of the Src family of kinases.
Species:
Human

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