NB120-4817 Src antibody

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Rabbit anti Human, Mouse, Rat Src


Product Description for Src

Rabbit anti Human, Mouse, Rat Src.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Src

Product Category Primary Antibodies
Quantity 0.05 ml
Synonyms Schmidt Ruppin A2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications C, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, based on the human sequence. The sequence is conserved in mouse (tyrosine 534), chicken (tyrosine 527) and frog (tyrosine 525).
Background Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylatedleading to full activation.
General Readings This product has been used in:Haas M et al. Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J Biol Chem 277:18694-702 (2002). PubMed PMID: 11907028
Suhr SM et al. Antisense oligodeoxynucleotide evidence that a unique osteoclastic protein-tyrosine phosphatase is essential for osteoclastic resorption. J Bone Miner Res 16:1795-803 (2001). PubMed PMID: 11585343
Gil-Henn H et al. Regulation of protein-tyrosine phosphatases alpha and epsilon by calpain-mediated proteolytic cleavage. J Biol Chem 276:31772-9 (2001). PubMed PMID: 11429406
Cheng A et al. Attenuation of adhesion-dependent signaling and cell spreading in transformed fibroblasts lacking protein tyrosine phosphatase-1B. J Biol Chem 276:25848-55 (2001). PubMed PMID: 11346638
Giusti AF et al. Evidence that fertilization activates starfish eggs by sequential activation of a Src-like kinase and phospholipase cgamma. J Biol Chem 275:16788-94 (2000). PubMed PMID: 10747984
Ma YC et al. Src tyrosine kinase is a novel direct effector of G proteins. Cell 102:635-46 (2000). PubMed PMID: 11007482
Sussman MA et al. Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1. J Clin Invest 105:875-86 (2000). PubMed PMID: 10749567
Maher PA Disruption of cell-substrate adhesion activates the protein tyrosine kinase pp60(c-src). Exp Cell Res 260:189-98 (2000). PubMed PMID: 11035913
Osusky M et al. Autophosphorylation of purified c-Src at its primary negative regulation site. J Biol Chem 270:25729-32 (1995). PubMed PMID: 7592753
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human, Chicken, Mouse and Rat. Not yet tested in other species.

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