NB120-5218 Src antibody

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Rabbit anti Human Src


Product Description for Src

Rabbit anti Human Src.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Src

Product Category Primary Antibodies
Quantity 0.05 ml
Synonyms Schmidt Ruppin A2
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide (Human).
Background The Src family of non-receptor tyrosine kinases is involved in the modulation of a wide variety of normal and pathogenic cellular processes including growth, development, differentiation, and carcinogenesis. These proteins contain a consensus structure comprised of one or more N-terminalSH2 and/or SH3 domains involved in protein-protein interactions, a central catalytic tyrosine kinase domain, and a short C-terminal domain. This C-terminal domain contains a tyrosine residue that, when phosphorylated by Csk tyrosine kinase, functions as a negative regulatory site (tyrosines 530, 531, 522, 505, and 508 in Src, Fyn, Hck, Lck, and Lyn, respectively) by providing a binding site for the N-terminal SH2 domain. The association of these two domains blocks access to a critical tyrosine residue in the activation loop, the phosphorylation of which is required for enzyme activation. When the C-terminal domain tyrosine residue is dephosphorylated, the catalytic domaintyrosine can be maximally phosphorylated leading to full kinase activation.
Concentration 0.5 mg/ml
General Readings General / background references:Young MA et al. Dynamic coupling between the SH2 and SH3 domains of c-Src and Hck underlies their inactivation by C-terminal tyrosine phosphorylation. Cell 105:115-26 (2001). PubMed PMID: 11301007
Hubbard SR & Till JH Protein tyrosine kinase structure and function. Annu Rev Biochem 69:373-98 (2000). PubMed PMID: 10966463
Egan C et al. Activation of Src in human breast tumor cell lines: elevated levels of phosphotyrosine phosphatase activity that preferentially recognizes the Src carboxy terminal negative regulatory tyrosine 530. Oncogene 18:1227-37 (1999). PubMed PMID: 10022129
Thomas SM & Brugge JS Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13:513-609 (1997). PubMed PMID: 9442882
Thomas SM & Brugge JS Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13:513-609 (1997). PubMed PMID: 9442882
Immunogen affinity purified
Buffer System:
Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Immunogen affinity purified
Aff - Purified
Cross-reacts with Human.Expected to cross-react with Mouse, Rat due to sequence homology.Not yet tested in other species.

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