AM00503PU-N SREBP2 antibody

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Mouse anti Human SREBP2 1C6


Product Description for SREBP2

Mouse anti Human SREBP2 1C6.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for SREBP2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms SREBP-2
Presentation Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 1C6
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Recombinant protein encoding C-terminal amino acids 833-1141 of human SREBP-2
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Mol. Wt. of Antigen: 125 kDa (precursor) and 60-70 kDa (cleaved)
Cellular Localization: Cytoplasmic and nuclear
Application Immunoprecipitation (Not verified).
Western Blotting: 1-2 µg/ml for 2 hrs at RT.
Positive Control: HeLa and IMR5 cells.
Background SREBP-1 and -2 are transcription factors which participate in the control of cholesterol homeostasis. SREBPs proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~ 480-500 amino acid, Nterminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes.
General Readings
  1. Wang, X., et al. (1995) J. Biol. Chem. 270:18044-18050.
  2. Wang, X., et al. (1996) EMBO J. 15:1012-1020.
  3. Sato, R., et al. (1994) J. Biol. Chem. 269:17267-17273.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
This antibody detects SREBP-2 at aa 833-1141.

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