discontinued

AM12115PU-N SSEA4 antibody

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25 µg / €320.00

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Mouse anti Human SSEA4 MC-813-70

Product Description for SSEA4

Mouse anti Human SSEA4 MC-813-70.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for SSEA4

Product Category Primary Antibodies
Quantity 25 µg
Synonyms Embryonic Stem Cell Marker, SSEA-4, Stage-Specific Embryonic Antigen-4
Presentation Aff - Purified
Reactivity Hu
Applications F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone MC-813-70
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human embryonal carcinoma cell line 2102Ep
Application Western blot.
Immunoprecipitation.
Flow (Cell Surface): 0.1 µg/10e6 cells.
Immunofluorescence/Immunocytochemistry.
Immunohistochemistry (Paraffin).
Background Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro, while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. Undifferentiated human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas and are characterized by the expression of stage specific embryonic antigens SSEA-1 and SSEA-3, TRA-2-39, TRA-2-54 and the high molecular weight glycoproteins TRA-1-60 and TRA-1-81. In addition, SSEA-1, SSEA-3 and SSEA-4 are markers that characterize embryonic stem (ES) and embryonic germ (EG) cells. Specifically, undifferentiated cells from the human ES cell line H7 express SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, but not SSEA-1. Interferon induces expression of SSEA-3 and SSEA-4 in EC cells without inhibiting their growth or inducing their differentiation.
Concentration 0.5 mg/ml
General Readings
  1. Kannagi, R., et al., EMBO J. 2: 2355 (1983)
  2. Gang E.J. et al. Blood. Feb 15;109(4):1743-51 (2007)
  3. Henderson, J.K., et al. Stem Cells. 20: 329 (2002)
  4. Müller T, et al. Hum Reprod. 2009 Feb 27 (2009)
Storage Store the antibody (in aliquots) at -20°C
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.05% BSA and 0.05% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated
following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

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