DP3127 Substance P antibody

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Rabbit anti Bovine, Feline, Human, Porcine, Rat Substance P


Product Description for Substance P

Rabbit anti Bovine, Feline, Human, Porcine, Rat Substance P.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Substance P

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms SP, Substance-P
Presentation Purified
Reactivity Bov, Fe, Hu, Por, Rt
Applications C, P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Synthetic substance P (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2) conjugated to KLH.

Format: This antibody is supplied as liquid in 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Application Immunoblot: 1:500 - 1:1000
Immunohistochemistry: 1:50 - 1:200
This antibody can be used on both frozen and formalin fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. Recommended positive control: Brain.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1 mg/ml
General Readings 1. Nicoll, R., et al., Ann. Rev. Neuroscience, 3: 227, 1980/
2. Erspamer, V., Trends Neurosci.ence, 4: 267, 1981.
3. Couture, R., and Regoli, D., Pharmacol., 25: 1, 1982.
4. Pernow, B., et al., Pharmacol. Rev., 35: 85, 1983.
5. Bartho, L. and Holzer, P. Neuroscience 16: 1, 1985.
6. Lembeck, F., in Substance P, Metabolism and Biological Actions,
Jordan, C., and Oehme, P., (eds), Taylor and Francis, London, pp 137, 1985.
7. Nakanishi, S., Trends Neuroscience, 9: 41, 1986
References : 8. Krause, J. et al, Proc. Natl. Acad. Sc. USA 84: 881, 1987.
9. Maggio, J. Ann. Rev. Physiol. 11: 13, 1988.
10. Quirion, R., and Dam, T., V. Reg. Peptides, 22: 18, 1988.
11. Shigmoto, R. et al, J. Biol. Chem. 265: 623, 1990.
12. Salter, M. and Henry, J. Neuroscience 43: 601, 1991.
13. Larsen
Storage Store the antibody at 4°C. Do not freeze!
Do not dilute the entire reconstituted solution at once, since the diluted solution may have reduced stability. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good diluent to use with most antibodies.
Shelf life: one year from despatch.
This antibody reacts with substance P conjugated to BSA in slot blots. This antibody cross-reacts with substance P fragment (7-11), (6-11) and to a lesser extent with substance P (1-7) and neurokini A conjugated to BSA in blot analysis. This antiserum does not cross-react with neurokinin B, neuropeptide Y, vasoactive intestinal peptide, calcitonin gene related peptide (CGRP) or somatostatin.
Reacts with Rat, Cat, Porcine, Bovine, Monkey, and Human brain.

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