AP21390AF-N Subtilisin antibody

See related secondary antibodies

Search for all "Subtilisin"

10 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Bacillus sp. Subtilisin


Product Description for Subtilisin

Rabbit anti Bacillus sp. Subtilisin.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Subtilisin

Product Category Primary Antibodies
Quantity 10 mg
Synonyms Subtilisin E, apr, aprA, aprE, sprE
Presentation Azide Free
Reactivity Bacillus sp.
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Subtilisin isolated and purified from Bacillus subtilis.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Recommended Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/6,000.
Background Subtilisin (serine endopeptidase) is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis.
Subtilisins belong to subtilases, a group of serine proteases that initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. They are physically and chemically well-characterized enzymes. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton. They can be obtained from soil bacteria, for example, Bacillus amyloliquefaciens. Subtilisins are secreted in large amounts from many Bacillus species.
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized purified Hyperimmune IgG fraction
Azide Free
Restore by adding 1 ml of sterile distilled water
Subtilisin from Bacillus subtilis.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Bacillus subtilis.
Gene ID 939313

Accessory Products

  • LinkedIn