discontinued

NBP1-77440 Superoxide Dismutase 1 antibody

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0.2 ml / €430.00

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Chicken anti var. Superoxide Dismutase 1

NBP1-77440

Product Description for Superoxide Dismutase 1

Chicken anti var. Superoxide Dismutase 1.
Presentation: FITC
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Superoxide Dismutase 1

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation FITC
Reactivity var.
Applications E, WB
Clonality Polyclonal
Host Chicken
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q71S88
Immunogen:
Synthetic peptide taken from human CTR1 protein corresponding to amino acid corresponding to 1-50 aa from N-terminal end. The peptide was conserved in several other species. The selected peptide was post-synthetically modified to achieve highest antigenicty before coupling to carrier protein using hetero bifunctional cross linker for immunogen preparation.
Background Kinetoplastid glycosomes contain a variety of metabolic activities, such as glycolysis, beta-oxidation of fatty acids, lipid biosynthesis, and purine salvage. Confinement and sequestration of all metabolic activities tend to protect other cellular components form oxidative damages caused by oxygen radicals produced during metabolism. In Leishmaniasis, an iron superoxide dismutase (FeSODB) is expressed in low levels in the early logarithmic promastigote stage and increases toward the stationary promastigote and amastigote stages. A second FeSODB2 enzymes was cloned from the same region but its expression was high in the early logarithmic promastigote stage and decreases toward the stationary promastigote and amastigote stages. Mutationin FeSODB1 results in significant reduction in parasite in human macrophages, suggesting that FeSODB1 plays an important role in parasite growth and survival by protecting glycosomes form superoxide toxicity (1). Other parasites like trypnasoma, overexpressing mitochondrial FeSOD (iron superoxide dismutase) were more resistant to the PCD stimulus, unambiguously indicating the participation of mitochondrial O2o? in the signaling process (2). The Leishmania FeSODB1 and FeSODB2 enzymes are cloned form L chagasi promastigote cDNA library using degenerate primersderived form conserved amino acids form the MnSOD and FeSOD, the two enzymes having high homology to FeSODB1 and FeSODB2 (3). Overexpression of these FeSODs in L. chagasi parasites also showed enhanced protection against the free radical generating agents, paraquat and nitroprusside.. FeSODB1 and B2 are highly homologous proteins with a MW of 25kDa (208aa). The FeSODB1/2-selective antibodies were generated against FeSOD protein. The FeSOD1/2-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The FeSOD1 antibodies label a protein of approximately 26kDa in Western blot using Western blot positive control for FeSOD1 (PC-FwSODB1). FeSODB1/2-selective antibodies are also available in FITC conjugated preparations for confocal and immunocytochemical analyses.
Concentration .55 mg/ml
Storage Store at -20C. Avoid freeze-thaw cycles.
Format
Label:
FITC
Purification:
Affinity purified
Buffer System:
Antibody stabilization buffer.

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