BM5086P Synaptopodin / SYNPO antibody

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50 µg / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat Synaptopodin / SYNPO G1D4


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  • BM5086P

Product Description for Synaptopodin / SYNPO

Mouse anti Bovine, Guinea Pig, Gerbil, Human, Mouse, Rat Synaptopodin / SYNPO G1D4.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Synaptopodin / SYNPO

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms KIAA1029
Presentation Aff - Purified
Reactivity Bov, Ge, GP, Hu, Ms, Rt
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone G1D4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Isolated Rat kidney glomeruli.
Application Immunofluorescence. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections (After microwave treatment). 
Working Dilutions: 1/5-1/10 for 1h at RT. 
Western blot: 0.1-0.5 µg/ml. 
The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Background Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Product Citations
Purchased from Acris:
  1. Lechler P, Wu X, Bernhardt W, Campean V, Gastiger S, Hackenbeck T, et al. The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney. Am J Pathol. 2007 Nov;171(5):1483-98. PubMed PMID: 17982126. (Free PMC Article available, 8 images available)
  2. Câmpean V, Karpe B, Haas C, Atalla A, Peters H, Rupprecht H, et al. Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis. Am J Physiol Renal Physiol. 2008 May;294(5):F1174-84. doi: 10.1152/ajprenal.00320.2007. Epub 2008 Feb 13. PubMed PMID: 18272601.
  3. Yates LL, Papakrivopoulou J, Long DA, Goggolidou P, Connolly JO, Woolf AS, et al. The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation. Hum Mol Genet. 2010 Dec 1;19(23):4663-76. doi: 10.1093/hmg/ddq397. Epub 2010 Sep 14. PubMed PMID: 20843830. (Free PMC Article available, 7 images available)
  4. Xu J, Chen J, Dong Z, Meyuhas O, Chen JK. Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy. Kidney Int. 2014 Sep 17. doi: 10.1038/ki.2014.302. PubMed PMID: 25229342.
Originator or purchased from resellers:
  1. Mundel P, Heid HW, Mundel TM, Krüger M, Reiser J, Kriz W. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes. J Cell Biol. 1997 Oct 6;139(1):193-204. PubMed PMID: 9314539. (Free PMC Article available, 11 images available)
General Readings
  1. Mundel P, Gilbert P, Kriz W. Podocytes in glomerulus of rat kidney express a characteristic 44 KD protein. J Histochem Cytochem. 1991 Aug;39(8):1047-56. PubMed PMID: 1856454.
  2. Mundel P, Kriz W. Structure and function of podocytes: an update. Anat Embryol (Berl). 1995 Nov;192(5):385-97. PubMed PMID: 8546330.
  3. Mundel P, Reiser J, Kriz W. Phenotypic conversion and differentiation of human and rat podocytes in vitro. J. Am. Soc. Nephrol. 1997 8:8978-705.
  4. Mundel P, Reiser J, Zúñiga Mejía Borja A, Pavenstädt H, Davidson GR, Kriz W, et al. Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines. Exp Cell Res. 1997 Oct 10;236(1):248-58. PubMed PMID: 9344605.
  5. Kobayashi N, Reiser J, Kriz W, Kuriyama R, Mundel P. Nonuniform microtubular polarity established by CHO1/MKLP1 motor protein is necessary for process formation of podocytes. J Cell Biol. 1998 Dec 28;143(7):1961-70. PubMed PMID: 9864367. (Free PMC Article available, 6 images available)
  6. Barisoni L, Kriz W, Mundel P, D'Agati V. The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy. J Am Soc Nephrol. 1999 Jan;10(1):51-61. PubMed PMID: 9890309.
  7. Kihara I, Yaoita E, Kawasaki K, Yamamoto T, Hara M, Yanagihara T. Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy. Histopathology. 1999 Jun;34(6):537-47. PubMed PMID: 10383699.
  8. Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, et al. Synaptopodin regulates the actin-bundling activity of alpha-actinin in an isoform-specific manner. J Clin Invest. 2005 May;115(5):1188-98. Epub 2005 Apr 1. PubMed PMID: 15841212. (Free PMC Article available, 7 images available)

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer. 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Affinity Chromatography on Protein A
Buffer System:
Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
Lyophilized purified Ig fraction
Aff - Purified
Restore with 1 ml distilled water
Species Reactivity
Species reactivity (tested):
Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.
The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. 
Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
Gene ID 11346

Accessory Products

Proteins and/or Positive Controls

Proteins for Synaptopodin / SYNPO (1 products)

Catalog No. Species Pres. Purity   Source  

Synaptopodin / SYNPO (transcript variant 3)

Synaptopodin / SYNPO Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
  OriGene Technologies, Inc.

Positive controls for Synaptopodin / SYNPO (1 products)

Catalog No. Species Pres. Purity   Source  

SYNPO overexpression lysate

SYNPO overexpression lysate
  OriGene Technologies, Inc.
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