SM321A T-Cell Receptor TCR alpha/beta antibody

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Mouse anti Monkey, Rat T-Cell Receptor TCR alpha/beta R73


Product Description for T-Cell Receptor TCR alpha/beta

Mouse anti Monkey, Rat T-Cell Receptor TCR alpha/beta R73.
Presentation: Azide Free
Product is tested for Frozen Sections, Functional assay, Immunoprecipitation, Flow Cytometry.

Properties for T-Cell Receptor TCR alpha/beta

Product Category Primary Antibodies
Quantity 1 mg
Presentation Azide Free
Reactivity Mky, Rt
Applications C, F, FN, IP
Clonality Monoclonal
Clone R73
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Rat T Blasts and Erythrocytes
Isotype control SM20A (for use in rat samples)
Application Flow Cytometry (use 10 µl of 1/50 - 1/100 diluted antibody to label 106 cells in 100 µl or 100 µl whole blood), Immunohistochemistry on frozen sections (1/10 - 1/100), Immunoprecipitation and Functional Studies (2, 3). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
General Readings
  1. Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G. A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1;169(1):73-86. PubMed PMID: 2783336. (Free PMC Article available)
  2. Colić M, Ilić V, Pavlović MD, Tamatani T, Miyasaka M. Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells. Dev Immunol. 1996;5(1):37-51. PubMed PMID: 8828010. (Free PMC Article available)
  3. Matsumoto Y, Tsuchida M, Hanawa H, Abo T. Successful prevention and treatment of autoimmune encephalomyelitis by short-term administration of anti-T-cell receptor alpha beta antibody. Immunology. 1994 Jan;81(1):1-7. PubMed PMID: 7510661. (Free PMC Article available)
  4. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110. PubMed PMID: 10889948.
Storage Store the antibody undiluted at -20°C only. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Azide Free
SM321A recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative.
This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (2).
Cross reacts with Cynomolgus monkey (4).

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