discontinued

AM05098PU-N T Cell Receptor (TCR) V beta 13.1 antibody

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0.1 mg / €530.00

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Mouse anti Human T Cell Receptor (TCR) V beta 13.1 Immu222

AM05098PU-N

Product Description for T Cell Receptor (TCR) V beta 13.1

Mouse anti Human T Cell Receptor (TCR) V beta 13.1 Immu222.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 13.1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-13.1, TCR Vb13.1
Presentation Purified
Reactivity Hu
Applications F
Clonality Monoclonal
Clone Immu222
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Murine T cell hybridoma transfected with human V beta-13.1 gene segment
Isotype control AM03110PU-N, SM12P
Application T-cell repertoire studies.
Fluorescent microscopy or flow cytometry: 2µg / 5 x 10e5 cells/tests or 100µl whole blood.
General Readings
  1. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40(1):27-36. PubMed PMID: 8206523.
  2. Li, Y., et al., (1991), :The genomic structure of human V Beta 6 TCR genes, J. Exp. Med., 174, 1537 - 1547.
  3. Kimura N, Toyonaga B, Yoshikai Y, Triebel F, Debre P, Minden MD, et al. Sequences and diversity of human T cell receptor beta chain variable region genes. J Exp Med. 1986 Sep 1;164(3):739-50. PubMed PMID: 3755748. (Free PMC Article available)
  4. Ferradini, L., et al., Studies on the human TCR alpha beta variable region genesll. Identification of four additional V beta subfamilies, Eur. J. Immunol., 21, 935 - 942.
  5. Posnett, D.N., et al., (1996), First human TcR monoclonal antibody workshop, The Immunologist, 4, 1, 5 - 8.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody at -20°C.
The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstituted form for up to 1 month at 2 - 8°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS containing 1mg/ml BSA
State:
Lyophilized purified Ig
Purified
Reconstitution:
Restore with 0.5ml of distilled water
Specificity
Specificity:
This antibody reacts to the variable beta-13.1 chain of the T cell receptor also called TCRBV13S1 according to the nomenclature from Wei et al. V beta-13.1 is a complex subfamily of the TCR. This antibody recognizes the V beta-13.1 member (HBP34 sequence). The sequence V beta-13.3 (IGRb14) is not recognized. The antibody was characterized on human T-cell clones and by cell sorting followed by molecular analysis of the sorted cells. All of 11 sequence analyzed were V beta-13.1 subfamily cannot be formally excluded. It was been recently shown that Immu222 could recognize V beta-13.4 (Clone 4.1 sequence) and V beta-13.6 (IGRb16 sequence) subsets. No crossreactivity was detected with any of 18 V beta specific antibodies (representing - 70% of the alpha/beta repertoire) on a cell line sorted from PBL and 100% positive with Immu222.
Species:
Human.

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