NB100-65434 T Cell Receptor (TCR) V beta 16 antibody

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Mouse anti Rat T Cell Receptor (TCR) V beta 16 HIS42


Product Description for T Cell Receptor (TCR) V beta 16

Mouse anti Rat T Cell Receptor (TCR) V beta 16 HIS42.
Presentation: Supernatant
Product is tested for Frozen Sections, Flow Cytometry, Immunoprecipitation, Paraffin Sections.

Properties for T Cell Receptor (TCR) V beta 16

Product Category Primary Antibodies
Quantity 2 ml
Synonyms TCR V beta-16, TCR Vb16
Presentation Supernatant
Reactivity Rt
Applications C, F, IP, P
Clonality Monoclonal
Clone HIS42
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

PVG RT7.2 Rat Thymocytes
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, immunoprecipitation, Immunohistochemistry-Frozen, Immunoprecipitation and Flow cytometry / FACS analysis, flow cytometry / FACS analysis , immunoprecipitation , Immunohistochemistry-Frozen 1:10
Background The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tissue culture supernatant
Buffer System:
0.09% Sodium Azide
Tissue culture supernatant
NB100-65434 detects a minor subset of T cells. The subpopulation of HIS 42 positive T cells contains both CD4 and CD8 positive cells, in the same ratio as found in the peripheral T cell population. When bound to Sepharose beads, HIS 42 induces T cell proliferation in the presence of IL-2. In contrast to lymph node T cells a number of thymocytes are found to express HIS 42 only in the cytoplasm or together with membrane expression. Most bright HIS 42 surface labelled thymocytes are also positive for MRC OX-44, a marker predominantly identifying mature thymocytes. SDS PAGE analysis of the membrane molecules immunoprecipitated by HIS 42 shows a band at 85kD. On reduction it separates to two bands at 35kD and 48kD. From this data it is concluded that HIS 42 is directed against a determinant on the rat T cell receptor, which is common to a small number of T cells. Preliminary data suggests that the determinant is found on the beta chain, associated with the Vbeta16.1 gene product.

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