discontinued

CL077P T Cell Receptor (TCR) V beta 4 antibody

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0.2 mg / €370.00

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Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4

CL077P

Product Description for T Cell Receptor (TCR) V beta 4

Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 4

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms TCR V beta-4, TCR Vb4
Presentation Purified
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CTVB4
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM15P, SM15PX
Application Flow cytometry.
Concentration 0.2 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1:500 dilution.
9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C in media B.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
General Readings
  1. Tomonari, K., lovering, E. and Spencer, S. 1990 Correlation between the Vb4+ CD8+ T cell population and the H-2d haplotype. Immunogenetics 31:333-339.
  2. Padula, S.J., Lingenheld, E.G., Stabach, P.R., Chou, C.J., Kono, D.H. and Clark, R.B. 1991 Identificaion of encephalitogenic Vb4-bearing T cells in SJL mice-further evidence for the V region disease hypothesis? J. Immunol. 146:879-883.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS and contains 0.09% sodium azide (NaN3) as a preservative.
State:
Liquid purified
Purified
Specificity
Specificity:
This monoclonal antibody reacts with TCR Vb4 bearing T cells. TCR Vb4 is expressed in most known mouse strains.
Species:
Mouse.

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