discontinued

AM05104RP-N T Cell Receptor (TCR) V beta 5.2 antibody

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50 Tests / €700.00

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Mouse anti Human T Cell Receptor (TCR) V beta 5.2 36213

AM05104RP-N

Product Description for T Cell Receptor (TCR) V beta 5.2

Mouse anti Human T Cell Receptor (TCR) V beta 5.2 36213.
Presentation: Phyco
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 5.2

Product Category Primary Antibodies
Quantity 50 Tests
Synonyms TCR V beta-5.2, TCR Vb5.2
Presentation Phyco
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 36213
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
1C1 V beta 5.2 positive cell line.
Application T-cell repertoire studies.
Flow cytometry: 20µl/5 x 10e5 cells/test or 100µl whole blood.
Concentration 12.5 µg/ml
General Readings
  1. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40(1):27-36. PubMed PMID: 8206523.
  2. Concannon P, Pickering LA, Kung P, Hood L. Diversity and structure of human T-cell receptor beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6598-602. PubMed PMID: 3489234. (Free PMC Article available)
  3. Kimura, N., et al., (1986), Sequence and diversity of human T cell receptor beta-chain variable region genes, J. Exp. Med., 164, 739 - 750.
  4. Ferradini L, Roman-Roman S, Azocar J, Michalaki H, Triebel F, Hercend T. Studies on the human T cell receptor alpha/beta variable region genes. II. Identification of four additional V beta subfamilies. Eur J Immunol. 1991 Apr;21(4):935-42. PubMed PMID: 1826889.
  5. Diu A, Romagné F, Genevée C, Rocher C, Bruneau JM, David A, et al. Fine specificity of monoclonal antibodies directed at human T cell receptor variable regions: comparison with oligonucleotide-driven amplification for evaluation of V beta expression. Eur J Immunol. 1993 Jul;23(7):1422-9. PubMed PMID: 8391986.
  6. Postnett, D.N., et al., (1996), First human TcR monoclonal antibody workshop, The Immunologist, 4, (1), 5 - 8.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Label:
Phycoerythrin
Phyco
Buffer System:
PBS containing 2mg/ml BSA and 0,09% sodium azide
State:
Liquid purified Ig
Specificity
Specificity:
This antibody reacts to variable beta 5.2 chain of the T-cell receptor also called TCRBV5S2 according to the nomenclature from Wei et al.. This antibody recognizes only the PL2.5 sequence. It does not recognize the V beta 5.1 [HPB51 sequence and the V beta 5.3 (IGRb08 sequence]. Recognition of other members of the V beta 5 family has not been detected but cannot be formally excluded. Cell sorting on PBL gives a heterogenous pattern of staining of the sorted cells. The antibody may thus be sensitive to the clonotypic environment (V alpha, J alpha, D beta, J beta) though molecular analysis of transcripts from the sorted V beta 5.2 positive cells shows defferent V alpha, D and J beta. The specificity of this antibody has been confirmed at the First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995.
Species:
Human.

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