AM05104RP-N T Cell Receptor (TCR) V beta 5.2 antibody

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Mouse anti Human T Cell Receptor (TCR) V beta 5.2 36213


Product Description for T Cell Receptor (TCR) V beta 5.2

Mouse anti Human T Cell Receptor (TCR) V beta 5.2 36213.
Presentation: Phyco
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 5.2

Product Category Primary Antibodies
Quantity 50 Tests
Synonyms TCR V beta-5.2, TCR Vb5.2
Presentation Phyco
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 36213
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

1C1 V beta 5.2 positive cell line.
Application T-cell repertoire studies.
Flow cytometry: 20µl/5 x 10e5 cells/test or 100µl whole blood.
Concentration 12.5 µg/ml
General Readings
  1. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40(1):27-36. PubMed PMID: 8206523.
  2. Concannon P, Pickering LA, Kung P, Hood L. Diversity and structure of human T-cell receptor beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6598-602. PubMed PMID: 3489234. (Free PMC Article available)
  3. Kimura, N., et al., (1986), Sequence and diversity of human T cell receptor beta-chain variable region genes, J. Exp. Med., 164, 739 - 750.
  4. Ferradini L, Roman-Roman S, Azocar J, Michalaki H, Triebel F, Hercend T. Studies on the human T cell receptor alpha/beta variable region genes. II. Identification of four additional V beta subfamilies. Eur J Immunol. 1991 Apr;21(4):935-42. PubMed PMID: 1826889.
  5. Diu A, Romagné F, Genevée C, Rocher C, Bruneau JM, David A, et al. Fine specificity of monoclonal antibodies directed at human T cell receptor variable regions: comparison with oligonucleotide-driven amplification for evaluation of V beta expression. Eur J Immunol. 1993 Jul;23(7):1422-9. PubMed PMID: 8391986.
  6. Postnett, D.N., et al., (1996), First human TcR monoclonal antibody workshop, The Immunologist, 4, (1), 5 - 8.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Buffer System:
PBS containing 2mg/ml BSA and 0,09% sodium azide
Liquid purified Ig
This antibody reacts to variable beta 5.2 chain of the T-cell receptor also called TCRBV5S2 according to the nomenclature from Wei et al.. This antibody recognizes only the PL2.5 sequence. It does not recognize the V beta 5.1 [HPB51 sequence and the V beta 5.3 (IGRb08 sequence]. Recognition of other members of the V beta 5 family has not been detected but cannot be formally excluded. Cell sorting on PBL gives a heterogenous pattern of staining of the sorted cells. The antibody may thus be sensitive to the clonotypic environment (V alpha, J alpha, D beta, J beta) though molecular analysis of transcripts from the sorted V beta 5.2 positive cells shows defferent V alpha, D and J beta. The specificity of this antibody has been confirmed at the First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995.

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