AM33394PU-N T Cell Receptor (TCR) V beta 8.1, 8.2 antibody

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Rat anti Mouse T Cell Receptor (TCR) V beta 8.1, 8.2 KJ16-133.18

Product Description for T Cell Receptor (TCR) V beta 8.1, 8.2

Rat anti Mouse T Cell Receptor (TCR) V beta 8.1, 8.2 KJ16-133.18.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 8.1, 8.2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-8.1, TCR V beta-8.2, TCR Vb8.1, TCR Vb8.2
Presentation Aff - Purified
Reactivity Ms
Applications F, IP
Clonality Monoclonal
Clone KJ16-133.18
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

TCR receptor from T cell hybridoma DO-11.10
Application Flow Cytometry: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. 
Immunoprecipitation: Reported in Litterature.
Background The TCR Vβ 8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. KJ16-133.18 reacts with Vβ8.1, 8.2 only, but not Vβ8.3. Most mouse strains have about 20% Vβ 8+ T cells in the periphery.However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ 8 family loci.Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vβ 8.1 cells in the periphery.Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ 8 and selectively eliminate those T cells in vivo.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Rieder SA, Nagarkatti P, Nagarkatti M. CD1d-independent activation of invariant natural killer T cells by staphylococcal enterotoxin B through major histocompatibility complex class II/T cell receptor interaction results in acute lung injury. Infect Immun. 2011 Aug;79(8):3141-8. doi: 10.1128/IAI.00177-11. Epub 2011 May 31. PubMed PMID: 21628519. (Free PMC Article available, 5 images available)
General Readings
  1. Sim GK, Augustin AA. V beta gene polymorphism and a major polyclonal T cell receptor idiotype. Cell. 1985 Aug;42(1):89-92. PubMed PMID: 2410144.
  2. Ignatowicz L, Kappler JW, Marrack P, Scherer MT. Identification of two V beta 7-specific viral superantigens. J Immunol. 1994 Jan 1;152(1):65-71. PubMed PMID: 8254207.
  3. Haskins K, Hannum C, White J, Roehm N, Kubo R, Kappler J, et al. The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype. J Exp Med. 1984 Aug 1;160(2):452-71. PubMed PMID: 6206178. (Free PMC Article available)
  4. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986 Feb;83(3):767-71. PubMed PMID: 3456168. (Free PMC Article available)
  5. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992;36(3):189-94. PubMed PMID: 1319398.
  6. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989 Jan 13;56(1):27-35. PubMed PMID: 2521300.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
PBS, pH 7.2
0.09% Sodium Azide
Liquid purified IgG fraction
Aff - Purified
This antibody recognizes the V beta 8.1 and V beta 8.2 determinants of the T cell receptor.

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