CL080P T Cell Receptor (TCR) V beta 8.3 antibody

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0.2 mg / €370.00

Quick Overview

Mouse anti Mouse T Cell Receptor (TCR) V beta 8.3 KT-8C1


Product Description for T Cell Receptor (TCR) V beta 8.3

Mouse anti Mouse T Cell Receptor (TCR) V beta 8.3 KT-8C1.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 8.3

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms TCR V beta-8.3, TCR Vb8.3
Presentation Purified
Reactivity Ms
Applications F
Clonality Monoclonal
Clone KT-8C1
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow Cytometry.
Concentration 0.2 mg/ml

1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1:500 dilution.
9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C in media B.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
General Readings
  1. Sanchez-Madrid F, Simon P, Thompson S, Springer TA. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602. PubMed PMID: 6193226. (Free PMC Article available)
  2. Springer T, Galfrè G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539-51. PubMed PMID: 81133.
  3. Springer T, Galfré G, Secher DS, Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301-6. PubMed PMID: 89034.
  4. Vignali DA, Bickle QD, Crocker P, Taylor MG. Antibody-dependent killing of Schistosoma mansoni schistosomula in vitro by starch-elicited murine macrophages. Critical role of the cell surface integrin Mac-1 in killing mediated by the anti-Mr 16,000 mAb B3A. J Immunol. 1990 May 15;144(10):4030-7. PubMed PMID: 2185318.
  5. Kantor AB, Stall AM, Adams S, Herzenberg LA, Herzenberg LA. Differential development of progenitor activity for three B-cell lineages. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3320-4. PubMed PMID: 1565622. (Free PMC Article available)
  6. Jutila MA, Kroese FG, Jutila KL, Stall AM, Fiering S, Herzenberg LA, et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. Eur J Immunol. 1988 Nov;18(11):1819-26. PubMed PMID: 2849552.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS and contains 0.09% sodium azide (NaN3) as a preservative.
Liquid purified
This monoclonal antibody detects TCR Vb 8.3 bearing T cells.

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