NB100-64288 T Cell Receptor (TCR) V beta 8.5 antibody

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Mouse anti Rat T Cell Receptor (TCR) V beta 8.5 B73


Product Description for T Cell Receptor (TCR) V beta 8.5

Mouse anti Rat T Cell Receptor (TCR) V beta 8.5 B73.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) V beta 8.5

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms TCR V beta-8.5, TCR Vb8.5
Presentation Purified
Reactivity Rt
Applications F
Clonality Monoclonal
Clone B73
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

T cell clone expressing the TCR Vb8.5 gene product
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Application flow cytometry / FACS analysis, This antibody is useful for flow cytometry / FACS analysis, flow cytometry / FACS analysis 1/50 - 1/100
Background The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
Concentration 1 mg/ml
General Readings
  1. Torres-Nagel NE, Gold DP, Hünig T. Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies. Immunogenetics. 1993;37(4):305-8. PubMed PMID: 8420840.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein A purified
Buffer System:
0.09% Sodium Azide
protein A purified
NB100-64288 is specific for the T-cell receptor V beta 8.5 gene product.4% of unseparated LEW rat lymph node cells label with this. Reactivity is restricted to ab T cells of which 6.5% were stained (1).

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