Product Description for T-Cell Receptor TCR Vb 11
Rat anti Mouse T-Cell Receptor TCR Vb 11 CTVB11.
Presentation: PE
Product is tested for Flow Cytometry.
Presentation: PE
Product is tested for Flow Cytometry.
Properties for T-Cell Receptor TCR Vb 11
Product Category | Primary Antibodies |
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Quantity | 50 µg |
Synonyms | TCR V beta11, TCR Vb11 |
Presentation | PE |
Reactivity | Ms |
Applications | F |
Clonality | Monoclonal |
Clone | CTVB11 |
Host | Rat |
Isotype | IgG2b |
Shipping to | Worldwide |
PDF datasheet | View Datasheet |
Manufacturer | Acris Antibodies GmbH |
Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
Isotype control | SM19R |
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Application | Flow cytometry. |
Concentration | 0.1 mg/ml |
Protocols | FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). |
General Readings |
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Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should protected from light. Shelf life: one year from despatch. |
Format |
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Specificity |
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Accessory Products
Recommended Secondary Antibodies for T-Cell Receptor TCR Vb 11 (11 products)
Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
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R1371AP |
Rat IgG (H+L chain) antibody |
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Rabbit | AP | |||||
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R1371B |
Rat IgG (H+L chain) antibody |
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Rabbit | Biotin | |||||
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R1371F |
Rat IgG (H+L chain) antibody |
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Rabbit | FITC | |||||
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R1371HRP |
Rat IgG (H+L chain) antibody |
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Rabbit | HRP | C, E, WB | ||||
Acris Antibodies GmbH | |||||||
R1371T |
Rat IgG (H+L chain) antibody |
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Rabbit | TRITC | |||||
Acris Antibodies GmbH | |||||||
R1413R |
Rat IgG (H+L chain), F(ab)2 Fragment antibody |
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Goat | PE | |||||
Acris Antibodies GmbH | |||||||
R1415R |
Rat IgG F(ab)2 specific (adsorbed F(ab)2 Fragment) antibody |
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Goat | PE | |||||
Acris Antibodies GmbH | |||||||
R1428R |
Rat IgG (H+L chain) F(ab')2 Fragment antibody |
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Donkey | PE | |||||
Acris Antibodies GmbH | |||||||
R1439C2 |
Rat IgG (H+L chain, adsorbed) antibody |
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Goat | Cy2 | |||||
Acris Antibodies GmbH | |||||||
R1439C3 |
Rat IgG (H+L chain, adsorbed) antibody |
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Goat | Cy3 | |||||
Acris Antibodies GmbH | |||||||
R1439C5 |
Rat IgG (H+L chain, adsorbed) antibody |
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Goat | Cy5 | |||||
Acris Antibodies GmbH |