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CL083R T-Cell Receptor TCR Vb 11 antibody

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Rat anti Mouse T-Cell Receptor TCR Vb 11 CTVB11

CL083R

Product Description for T-Cell Receptor TCR Vb 11

Rat anti Mouse T-Cell Receptor TCR Vb 11 CTVB11.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for T-Cell Receptor TCR Vb 11

Product Category Primary Antibodies
Quantity 50 µg
Synonyms TCR V beta11, TCR Vb11
Presentation PE
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CTVB11
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM19R
Application Flow cytometry.
Concentration 0.1 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
General Readings
  1. Tomonari, K. and Lovering, E. 1989 T cell receptor-specific monoclonal antibodies against a Vβ11-positive mouse T cell clone. Immunogentics 28:445-451.
  2. Pircher, H., Ohashi, P., Miescher, G., Lang, R., Zikopoulos, A., Burki, K., Mak, T. W., MacDonald, H.R. and Hengartner, H. 1990 T cell receptor (TCR) β chain transgenic mice: studies on allelic exclusion and the TCR+ gamma/delta population. Eur. J. Immunol. 20:417-424.
  3. Bill J, Kanagawa O, Woodland DL, Palmer E. The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells. J Exp Med. 1989 Apr 1;169(4):1405-19. PubMed PMID: 2538552. (Free PMC Article available)
  4. Gao, E., Kanagawa, O. and Sprent, J. 1989 Capacity of unprimed CD4+ and CD8+ T cells expressing Vβ11 receptors to respond to I-E alloantigens in vivio. J. Exp Med. 170: 1947-1957.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should protected from light.
Shelf life: one year from despatch.
Format
Label:
PE
Buffer System:
PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
State:
Liquid, purified.
Specificity
Specificity:
This monoclonal antibody reacts with the TCR Vβ11 bearing T cell. TCR Vβ11 may be deleted in mouse strains expressing the MHC class II I-E antigens.
Species:
Mouse.

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