discontinued

CL084B T Cell Receptor (TCR) Vb 12 antibody

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0.1 mg / €300.00

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Rat anti Mouse T Cell Receptor (TCR) Vb 12 CTVB12b

Product Description for T Cell Receptor (TCR) Vb 12

Rat anti Mouse T Cell Receptor (TCR) Vb 12 CTVB12b.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 12

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-12, TCR Vb12
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CTVB12b
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM15B
Application Flow cytometry.
Concentration 0.1 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (Streptavidin-PE) at a 1:50 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: C3H.SW
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: Biotin Rat IgG2a
General Readings
  1. Tomonari K. Tcrb-V12-specific superantigens encoded by mouse mammary tumor proviruses. Immunogenetics. 1994;39(1):65-7. PubMed PMID: 7693584.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
State:
Liquid, purified.
Specificity
Specificity:
This monoclonal antibody reacts with TCR Vb12b bearing cells.
Species:
Mouse.

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