AM05099FC-N T Cell Receptor (TCR) Vb 17 antibody

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Mouse anti Human T Cell Receptor (TCR) Vb 17 E17.5F3


Product Description for T Cell Receptor (TCR) Vb 17

Mouse anti Human T Cell Receptor (TCR) Vb 17 E17.5F3.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 17

Product Category Primary Antibodies
Quantity 50 Tests
Synonyms TCR V beta-17, TCR Vb17
Presentation FITC
Reactivity Hu
Applications F
Clonality Monoclonal
Clone E17.5F3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mouse T-cell hybridoma transfected with V beta-17 gene segment.
Hybridization of X63 Ag 8.653.
Mmyeloma cells with spleen cells from BALB/c mice.
Isotype control SM10F (for use in human samples)
Application Studies have shown that V beta-17 may be useful in T-cell repertoire research.
Superantigenic stimulation of T cells; V beta-17 seems to be the target of MAM (6).
Flow Cytometry: 20 µl/5x10e5 cells/test.
General Readings
  1. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40(1):27-36. PubMed PMID: 8206523.
  2. Kimura N, Toyonaga B, Yoshikai Y, Du RP, Mak TW. Sequences and repertoire of the human T cell receptor alpha and beta chain variable region genes in thymocytes. Eur J Immunol. 1987 Mar;17(3):375-83. PubMed PMID: 3494611.
  3. Diu A, Romagné F, Genevée C, Rocher C, Bruneau JM, David A, et al. Fine specificity of monoclonal antibodies directed at human T cell receptor variable regions: comparison with oligonucleotide-driven amplification for evaluation of V beta expression. Eur J Immunol. 1993 Jul;23(7):1422-9. PubMed PMID: 8391986.
  4. Romagné F, Besnardeau L, Malissen B. A versatile method to produce antibodies to human T cell receptor V beta segments: frequency determination of human V beta 2+ T cells that react with toxic-shock syndrome toxin-1. Eur J Immunol. 1992 Oct;22(10):2749-52. PubMed PMID: 1396978.
Storage Store the antibody undiluted (in the Dark) at 2-8°C.
Shelf life: one year from despatch.
Buffer System:
PBS containing 2 mg/ml BSA as stabilizer and 0.09% Sodium Azide as preservative
Liquid IgG fraction
This antibody recognizes Human variable beta-17 chain of the T-cell receptor also called TCRBV17S1 according to the nomenclature of Wei et al.(1). V beta-17 is a single membered family (HBVT02). This antibody has been further characterized by cell sorting on PBL using this antibody followed by analysis of sorted cells by molecular biology. Analysis of alpha chain mRNA by PCR with a panel of a specific oligonucleotides shows transcripts for most V alpha sequences. Analysis of beta chain mRNA by anchored PCR and sequencing, only shows transcripts for beta-17 gene segment (HBVT02 sequence). This antibody is described in Reference 4.
The specificity of this antibody has been confirmed at the First Human TCR Monoclonal Antibody Workshop in San Francisco in 1995 (5).

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