AM31622RP-N T Cell Receptor (TCR) Vb 20 antibody

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Mouse anti Human T Cell Receptor (TCR) Vb 20 ELL1.4


Product Description for T Cell Receptor (TCR) Vb 20

Mouse anti Human T Cell Receptor (TCR) Vb 20 ELL1.4.
Presentation: Phyco
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 20

Product Category Primary Antibodies
Quantity 50 Tests
Synonyms TCR V beta-20, TCR Vb20
Presentation Phyco
Reactivity Hu
Applications F
Clonality Monoclonal
Clone ELL1.4
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Mouse T-cell clone hybridoma transfected with human V beta 20 gene segment
Application T-cell repertoire studies.
Flow cytometry: 20 µl/5 x 10e5 cells/test or 100 µl whole blood.
Since this antibody recognizes a small cell population, it is often preferable to use double staining experiments with another T cell marker (CD2, CD3, CD4, CD8, etc.). Double staining is also possible with the purified unlabelled form.
General Readings
  1. Wei S, Charmley P, Robinson MA, Concannon P. The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 1994;40(1):27-36. PubMed PMID: 8206523.
  2. Leiden, J.M., Strominger, J.L., Generation of diversity of the beta chain of the human T lymphocyte receptor for antigen,1986, Proc. Natl. Acad. Sci. USA 83, 4456 - 4460.
  3. Wilson, R.K., Lai E., Concannon, P., Barth, R.K., Hood, L.E., Structure, organisation and polymorphism of murine and human TCR alpha and beta chain gene families, 1998, Immunol. Rev., 101, 149 - 712.
  4. Charmley P, Wang K, Hood L, Nickerson DA. Identification and physical mapping of a polymorphic human T cell receptor V beta gene with a frequent null allele. J Exp Med. 1993 Jan 1;177(1):135-43. PubMed PMID: 7678110. (Free PMC Article available)
  5. Postnett, D.N., et al., First human TcR monoclonal antibody workshop, 1996, The Immunologist, 4, (1), 5 - 8.
Storage Store the antibody undiluted (in the Dark) at 2-8°C.
Shelf life: one year from despatch.
Ion Exchange Chromatography
Buffer System:
PBS containing 2 mg/ml BSA and 0.09% Sodium Azide
Liquid purified Ig fraction
This antibody reacts to variable beta 20 chain of the T-cell receptor also called TCRBV20S1 according to the nomenclature from Wei et al.. V beta 20 is a single membered subfamily (represented by the sequence HUT 102. This sequence is sometimes referred to as Vbeta18. This antibody has been characterized by cell sorting of PBL with ELL 1.4 followed by molecular biology analysis. In 8 sequences analyzed, only J beta 2.7 and J beta 1.1 were found associated with V beta 20. The N regions were different in each case. This specificity of this antibody has been confirmed at he First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995.

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