CL077B T Cell Receptor (TCR) Vb 4 antibody

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Rat anti Mouse T Cell Receptor (TCR) Vb 4 CTVB4

Product Description for T Cell Receptor (TCR) Vb 4

Rat anti Mouse T Cell Receptor (TCR) Vb 4 CTVB4.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 4

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-4, TCR Vb4
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone CTVB4
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow cytometry: Blocking Fc receptors with an anti-mouse CD16/32 mAb can significantly reduce background staining by the CTVB4 mAb.
Concentration 0.1 mg/ml

1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of (Streptavidin-PE) at a 1:50 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
(Representative Dot Blot)
Mouse Strain: C3H.SW
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: Biotin Rat IgG2a
General Readings
  1. Tomonari, K., et al. 1990. Correlation between the Vβ4+ CD8+ T cell population and the H-2d haplotype. Immunogenetics 31:333-339.
  2. Padula, S.J., et al. 1991. Identification of encephalitogenic Vβ4-bearing T cells in SJL mice-further evidence for the V region disease hyopothesis J. Immunol. 146:879-883.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Liquid purified
This monoclonal antibody reacts with TCR Vβ4 bearing T cells. TCR Vβ4 is expressed in most known mouse strains.

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