SM088B T Cell Receptor (TCR) Vb 7 antibody

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0.1 mg / €300.00

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Rat anti Mouse T Cell Receptor (TCR) Vb 7 TR310

Product Description for T Cell Receptor (TCR) Vb 7

Rat anti Mouse T Cell Receptor (TCR) Vb 7 TR310.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 7

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-7, TCR Vb7
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone TR310
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM19B
Application Flow cytometry.
Concentration 0.1 mg/ml

1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 µl of secondary antibody (Streptavidin-PE) at a 1:50 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 µl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
(Representative dot blot)
Mouse Strain: C3H.SW
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: Biotin Rat IgG2b
General Readings
  1. Okada CY, Holzmann B, Guidos C, Palmer E, Weissman IL. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990 May 1;144(9):3473-7. PubMed PMID: 1691759.
  2. Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993 Jan 15;150(2):683-94. PubMed PMID: 7678281.
  3. Ignatowicz, L., Kappier, J. W. Marrack, P. and Scherer, M. T. 1994 identification of two Vβ7-specific viral superantigens J. Immunol. 152:65-71.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS, 0.02% sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Liquid purified
This anti-mouse T cell receptor Vβ7 antigen monoclonal antibody reacts with TCR Vβ7 bearing T cells1. The TCR Vβ7 may be deleted in mouse strains expressing MlS-1a haplotype.

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