CL081F T-Cell Receptor TCR Vb 8.1, 8.2 antibody

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Mouse anti Mouse T-Cell Receptor TCR Vb 8.1, 8.2 KT-8E

Product Description for T-Cell Receptor TCR Vb 8.1, 8.2

Mouse anti Mouse T-Cell Receptor TCR Vb 8.1, 8.2 KT-8E.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for T-Cell Receptor TCR Vb 8.1, 8.2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta8, TCR Vb8
Presentation FITC
Reactivity Ms
Applications F
Clonality Monoclonal
Clone KT-8E
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application This antibody is suitable for use in Flow cytometry (use 1 µg to label 10e6 cells).
Concentration 0.1 mg/ml

1. Prepare a cell suspension in media A. For cell preparations, deplete the red lood cell population.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add 1 µg* of CL081F per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results-Tissue Distribution:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1 µg/10e6 cells
Isotypic Control: FITC Mouse IgG2a

Cell Source: Thymus
Percentage of cells stained above control: 6.1%

N.B. Appropriate control samples should always be included in any labelling studies.
* For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
General Readings
  1. Tomonari, K., Immunogenti (in press)
Storage Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Buffer System:
PBS with 0.09% Sodium azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Liquid purified IgG fraction.
Reacts with with mouse TCR Vb 8.2, 8.3, bearing T cells.

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