SM089B T Cell Receptor (TCR) Vb 8.1, 8.2 antibody

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Rat anti Mouse T Cell Receptor (TCR) Vb 8.1, 8.2 KJ16


Product Description for T Cell Receptor (TCR) Vb 8.1, 8.2

Rat anti Mouse T Cell Receptor (TCR) Vb 8.1, 8.2 KJ16.
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for T Cell Receptor (TCR) Vb 8.1, 8.2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms TCR V beta-8.1, TCR V beta-8.2, TCR Vb8.1, TCR Vb8.2
Presentation Biotin
Reactivity Ms
Applications F
Clonality Monoclonal
Clone KJ16
Host Rat
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Ag/MHC receptor from DO-11.10, specific for chicken ovalbumin and I-Ad 3
Application Flow cytometry: single, dual, or three colour.
(Reported to be useful for Immunoprecipitation.)
Background V beta 8.1, 8.2 T-cell receptor (TCR) antigen is expressed by 15-20% of peripheral T cells in most strains of mice (ie. Balb/c, AKR, C57BL/6, B10.A), and with a number of T cell hybridomas (ie. DO-11.10, 3DT-52.2, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4).
General Readings
  1. Sim GK, Augustin AA. V beta gene polymorphism and a major polyclonal T cell receptor idiotype. Cell. 1985 Aug;42(1):89-92. PubMed PMID: 2410144.
  2. Ignatowicz, L., Kappler, J.W., Marrack, P., and Sherer, M.T. 1994. Identification of two Vβ7-specific viral superantigens. J. Immunology. 152:65.
  3. Haskins K, Hannum C, White J, Roehm N, Kubo R, Kappler J, et al. The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype. J Exp Med. 1984 Aug 1;160(2):452-71. PubMed PMID: 6206178. (Free PMC Article available)
  4. Yagüe J, White J, Coleclough C, Kappler J, Palmer E, Marrack P. The T cell receptor: the alpha and beta chains define idiotype, and antigen and MHC specificity. Cell. 1985 Aug;42(1):81-7. PubMed PMID: 2410143.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS, 0.09% sodium azide (NaN3) and a highly purified grade of BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Liquid purified Ig
This antibody reacts with the mouse V beta 8.1, 8.2 T-cell receptor (TCR) antigen.
1 µg is the maximum amount of antibody required for 1 x 10e6 cells. Lesser amounts of antibody may be sufficient and it is recommended that each investigator determine dilutions appropriate for individual use. Appropriate controls should always be included in any labeling study. This clone has been reported to stimulate IL-2 production by DO-11.10, 3DT-52.2, and 51.4 cells and to block responses of DO-11.10, SKK-9.11, and D1G10G113.

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