SM612P T Cells / Thymocytes antibody

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Mouse anti Rabbit T Cells / Thymocytes KEN-5

Product Description for T Cells / Thymocytes

Mouse anti Rabbit T Cells / Thymocytes KEN-5.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for T Cells / Thymocytes

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms T Lymphocytes, T-Cells, T-lymphocytes
Presentation Purified
Reactivity Rb
Applications C, F, IP
Clonality Monoclonal
Clone KEN-5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Rabbit Thymocytes.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse PA1 myeloma cell line.
Isotype control AM03095PU-N
Application Immunoprecipitation.
Immunohistochemistry frozen.
Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
General Readings
  1. Kotani M, Yamamura Y, Tamatani T, Kitamura F, Miyasaka M. Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens. J Immunol Methods. 1993 Jan 4;157(1-2):241-52. PubMed PMID: 7678632.
  2. Raman C, Knight KL. CD5+ B cells predominate in peripheral tissues of rabbit. J Immunol. 1992 Dec 15;149(12):3858-64. PubMed PMID: 1281192.
  3. Dewals B, Boudry C, Farnir F, Drion PV, Vanderplasschen A. Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection. PLoS One. 2008 Feb 20;3(2):e1627. doi: 10.1371/journal.pone.0001627. PubMed PMID: 18286184. (Free PMC Article available, 4 images available)
  4. Guerrero I, Ferrian S, Blas E, Pascual JJ, Cano JL, Corpa JM. Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms. Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):75-81. doi: 10.1016/j.vetimm.2010.11.017. Epub 2010 Dec 1. PubMed PMID: 21190737.
  5. Gillet L, Schroeder H, Mast J, Thirion M, Renauld JC, Dewals B, et al. Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity. Vet Res. 2009 Sep-Oct;40(5):51. doi: 10.1051/vetres/2009034. Epub 2009 Jun 18. PubMed PMID: 19531344. (Free PMC Article available, 5 images available)
  6. Matsumura T, Kugiyama K, Sugiyama S, Ota Y, Doi H, Ogata N, et al. Suppression of atherosclerotic development in Watanabe heritable hyperlipidemic rabbits treated with an oral antiallergic drug, tranilast. Circulation. 1999 Feb 23;99(7):919-24. PubMed PMID: 10027816.
  7. Hoefer IE, Grundmann S, van Royen N, Voskuil M, Schirmer SH, Ulusans S, et al. Leukocyte subpopulations and arteriogenesis: specific role of monocytes, lymphocytes and granulocytes. Atherosclerosis. 2005 Aug;181(2):285-93. PubMed PMID: 16039282.
  8. Gu W, Holland M, Janssens P, Seamark R, Kerr P. Immune response in rabbit ovaries following infection of a recombinant myxoma virus expressing rabbit zona pellucida protein B. Virology. 2004 Jan 20;318(2):516-23. PubMed PMID: 14972520.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing
Shelf life: One year from despatch.
Affinity Chromatography on Protein G
Buffer System:
0.09% Sodium Azide
Liquid purified IgG fraction from tissue culture supernatant
This antibody recognises Rabbit T lymphocytes and immunoprecipitates, a 67kD glycoprotein.
This antibody was initially reported as recognising Rabbit CD5 (Ref.1), but does not bind to Rabbit CD5 transfectants.
Known Rabbit CD5 antibodies also show binding to most B lymphocytes (Ref.2), which are not labelled by this clone.

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