AP00958PU-N t-PA / PLAT antibody

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Sheep anti Rabbit t-PA / PLAT

Product Description for t-PA / PLAT

Sheep anti Rabbit t-PA / PLAT.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for t-PA / PLAT

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Alteplase, Reteplase, Tissue-type plasminogen activator, tPA
Presentation Purified
Reactivity Rb
Applications E, WB
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Recombinant rabbit tPA
Application ELISA: 1:100,000.
Western blot: 1 - 5 µg/ml.
Background TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, cell migration and many other physiopathological events. TPA binds to fibrin with high affinity; this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.
Storage Store the antibody at -70°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
0.05M Sodium phosphate, pH 6.6 containing 0.1M Sodium chloride and 1mM EDTA
Liquid purified Ig
This antibody recognizes single and two-chain Rabbit tPA, both alone and in complex under reducing and native conditions.

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