NB600-377 T7 Epitope Tag (MASMTGGQQMG) antibody

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0.1 ml / €380.00
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Goat anti T7 Epitope Tag (MASMTGGQQMG)

NB600-377

Product Description for T7 Epitope Tag (MASMTGGQQMG)

Goat anti T7 Epitope Tag (MASMTGGQQMG).
Presentation: HRP
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for T7 Epitope Tag (MASMTGGQQMG)

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation HRP
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Goat
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Goats were immunized with T7 cleavage site (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). Molar enzyme/antibody protein ratio is 4:1.
Application ELISA, Immunocytochemistry, immunohistochemistry, Western Blot, Suggested working dilutions: * ELISA 1:10000-1:100000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Histo/cyto 1:200-1:500 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, immunohistochemistry and immunocytochemistry. , ELISA 1:10000-1:100000, immunohistochemistry, Western Blot 1:1000-1:30000, Immunocytochemistry 1:200-1:500,
Background The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
Concentration 1 mg/ml
Storage Store at 4C. Do not freeze.
Format
Label:
HRP
Purification:
Immunogen affinity purified
Buffer System:
0.1% Pro-Clean 400
Purity:
Immunogen affinity purified
Specificity
Specificity:
Antibody recognizes the T7 cleavage site.

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