AM33340PU-N TAG72 antibody

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0.2 mg / €390.00
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Quick Overview

Mouse anti Bovine, Canine, Human, Rat TAG72 CA72/733

Product Description for TAG72

Mouse anti Bovine, Canine, Human, Rat TAG72 CA72/733.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections, Paraffin Sections.

Properties for TAG72

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms CA 72-4, CA72-4, Tumor-Associated Glycoprotein 72, sialy-Tn
Presentation Purified
Reactivity Bov, Can, Hu, Rt
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone CA72/733
Host Mouse
Isotype IgG1
Molecular weight 220kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Recombinant Human TAG-72 protein.
Isotype control SM20P (for use in rat samples)
Application ELISA: Use Antibody without BSA for coating.
Western Blot: 0.5-1 µg/ml. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. 
Positive Control: Jurkat cells, Breast or lung carcinoma.
Background Tumor-associated glycoprotein (TAG) 72 is a tumor marker, identified and characterized using two different monoclonal antibodies B72.3 and CC49. It is a mucin-like protein of high molecular weight (220-400 kd). The protein is expressed in several epithelial-derived cancers including 94% of colonic adenocarcinomas, 84% of invasive ductal carcinomas of the breast, 96% of non-small cell lung carcinomas, 100%of common epithelial ovarian carcinomas, as well as the majority of pancreatic, gastric, and esophageal cancers evaluated. The protein expression is also detected in fetal colon, stomach, and esophagus, thus defining TAG-72 as an oncofetal antigen.
Concentration 0.2 mg/ml
General Readings
  1. Lottich SC, Johnston WW, Szpak CA, Delong ER, Thor A, Schlom J. Tumor-associated antigen TAG-72: correlation of expression in primary and metastatic breast carcinoma lesions. Breast Cancer Res Treat. 1985;6(1):49-56. PubMed PMID: 2996665.
  2. Thor A, Ohuchi N, Szpak CA, Johnston WW, Schlom J. Distribution of oncofetal antigen tumor-associated glycoprotein-72 defined by monoclonal antibody B72.3. Cancer Res. 1986 Jun;46(6):3118-24. PubMed PMID: 3516392.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human, Cow, Dog, and Rat.
Specificity
Specificity:
This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin.
This Monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Cellular Localization: Cell surface and cytoplasmic.

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