TA311188 TET2 antibody

Goat Anti-TET2 Antibody

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0.1 mg / €325.00
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Quick Overview

Goat anti Canine, Human, Mouse TET2

Product Description for TET2

Goat anti Canine, Human, Mouse TET2.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections.

Properties for TET2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms KIAA1546, Methylcytosine dioxygenase TET2
Presentation Aff - Purified
Reactivity Can, Hu, Ms
Applications E, P
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6N021
Immunogen:
Peptide with sequence from the internal region of the protein sequence according to NP_001120680.1 and NP_060098.3.
AA Sequence:
C-PHPQSNNDQQREGSF
GeneID:
54790
Application Peptide ELISA: 1/16000 (Detection Limit).
Immunohistochemistry on Paraffin Sections: 2-6 µg/ml.
In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. 
This antibody has also been used successfully on Human Brain sections (See Orr et al, PLoS One. 2012;7(7):e41036. 2012 Jul 19. PMID: 22829908).
Background TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
It Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.
TET2 is Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Orr BA, Haffner MC, Nelson WG, Yegnasubramanian S, Eberhart CG. Decreased 5-hydroxymethylcytosine is associated with neural progenitor phenotype in normal brain and shorter survival in malignant glioma. PLoS One. 2012;7(7):e41036. doi: 10.1371/journal.pone.0041036. Epub 2012 Jul 19. PubMed PMID: 22829908. (Free PMC Article available, 7 images available)
General Readings
  1. Langemeijer SM, Kuiper RP, Berends M, Knops R, Aslanyan MG, Massop M, et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nat Genet. 2009 Jul;41(7):838-42. doi: 10.1038/ng.391. Epub 2009 May 31. PubMed PMID: 19483684.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Canine (Dog).
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001120680.1 and NP_060098.3).

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