NB100-75409 TFIIF antibody

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Chicken anti Human, Mouse, Rat TFIIF


Product Description for TFIIF

Chicken anti Human, Mouse, Rat TFIIF.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for TFIIF

Product Category Primary Antibodies
Quantity 0.05 mg
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The immunogen for this antibody was a sequence from 1a.a. to 249a.a. from TFIIF(a 249a.a. long protein)
Application Western Blot
Background The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD.
TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
General Readings
  1. Heng HH, Xiao H, Shi XM, Greenblatt J and Tsui LC., et al. Genes encoding general initiation factors for RNA polymerase II transcription are dispersed in the human genome
  2. Horikoshi M, Fujita H, Wang J, Takada R and Roeder RG., et al. Nucleotide and amino acid sequence of RAP30
  3. Sopta,M., Burton,Z.F. and Greenblatt,J., et al. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II
  4. , Horikoshi M., Fujita H., Wang J., Takada R., Roeder R.G. Nucleotide and amino acid sequence of RAP30.
  5. , Sopta M., Burton Z.F., Greenblatt J. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
  6. , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
  7. , Groft C.M., Uljon S.N., Wang R., Werner M.H. Structural homology between the Rap30 DNA-binding domain and linker histone H5: implications for preinitiation complex assembly.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
Phosphate-Buffered Saline.
Immunogen affinity purified
Aff - Purified
This antibody is reactive against Human, mouse, rat.

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