SM5021 THRA / THRB TR alpha-1/beta-1 antibody

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Mouse anti Human THRA / THRB C3

Product Description for THRA / THRB

Mouse anti Human THRA / THRB C3.
Properties: TR alpha-1/beta-1
Presentation: Ascites
Product is tested for Electrophoretic Mobility Shift Assay, Immunoprecipitation, Western blot / Immunoblot.

Properties for THRA / THRB

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms THRA
Presentation Ascites
Reactivity Hu
Applications EMSA, IP, WB
Clonality Monoclonal
Clone C3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified fragment of human TR beta-1 corresponding to amino acid residues 201-456.
Property TR alpha-1/beta-1
Application Gel Shift.
Immunoprecipitation: SM5021 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli.
Western Blot: 1/1000,detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli.
Background Thyroid hormone receptors (TR) are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1.
General Readings
  1. J. Biol. Chem., Nov 2000; 275: 38032.
  2. Endocrinology, Apr 2007; 148: 1764 - 1773
  3. Mol. Cell. Biol., Nov 2000; 20: 8329 - 8342.
  4. Mol Endocrinology 18(7), 1631-1642, Jul 2004
  5. Molecular Endocrinology 10.1210/me.2003-0289 2005
  6. JBC 2005, manuscript number M411514200.
  7. Biochem. Biophys. Res. Comm., 195(1): 385-392, 1993.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS containing 0.05% sodium azide as preservative.
Liquid diluted ascites.
SM5021 detects thyroid hormone receptor (TR) from human tissues.
This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1.
This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.
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