AM00408AF-N Thrombospondin antibody

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Mouse anti Human Thrombospondin MBC200.1


Product Description for Thrombospondin

Mouse anti Human Thrombospondin MBC200.1.
Presentation: Azide Free
Product is tested for Functional assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Thrombospondin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Azide Free
Reactivity Hu
Applications FN, ICC/IF, IP, WB
Clonality Monoclonal
Clone MBC200.1
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Native purified human TSP from platelets, N-terminal heparin-binding domain.
Mol. Wt. of Antigen: ~450kDa (non-reduced) 170 to 180 kDa (reduced)
Add. information Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, fibrillar extracellular matrix surrounding the cells.
Application Blocks Binding of TSP-1: 5-10 µg/ml.
Immunoprecipitation (Native verified): use Protein G, 2 µg/mg protein lysate.
Western Blotting: 1-2 µg/ml for 2 hrs at RT.
Not suitable for Immunohistology.
Background Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Concentration 1 mg/ml
General Readings
  1. Gupta K, et al. (1998) Biochim Biophys Acta, 61789:1-11.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4
Liquid ascites fluid
Azide Free
This antibody detects Thrombospondin.
It reacts with a 25 kDa peptide (heparin-binding domain) from thermolysin digests of TSP that is not disulfide bonded to any other region of the TSP molecule. Heparin efficiently inhibits the binding to TSP.

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