discontinued

AM00408PU-N Thrombospondin antibody

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0.1 mg / €480.00

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Mouse anti Human Thrombospondin MBC200.1

AM00408PU-N

Product Description for Thrombospondin

Mouse anti Human Thrombospondin MBC200.1.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Thrombospondin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone MBC200.1
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Native purified human TSP from platelets, N-terminal heparin-binding domain.
Remarks:
Mol. Wt. of Antigen: ~450kDa (non-reduced) 170 to 180 kDa (reduced)
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum, fibrillar extracellular matrix surrounding the cells.
Application Immunofluorescence.
Immunoprecipitation (Native verified): use Protein G, 2 µg/mg protein lysate.
Western Blotting: 1-2 µg/ml for 2 hrs at RT.
Not suitable for Immunohistology.
Background Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.
Concentration 0.2 mg/ml
General Readings
  1. Gupta K, et al. (1998) Biochim Biophys Acta, 61789:1-11.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
State:
Liquid ascites fluid
Purified
Specificity
Specificity:
This antibody detects Thrombospondin.
It reacts with a 25 kDa peptide (heparin-binding domain) from thermolysin digests of TSP that is not disulfide bonded to any other region of the TSP molecule. Heparin efficiently inhibits the binding to TSP.
Species:
Human.

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