DP3130 Thrombospondin antibody

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Rabbit anti Bovine, Human, Mouse Thrombospondin


Product Description for Thrombospondin

Rabbit anti Bovine, Human, Mouse Thrombospondin.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Thrombospondin

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Reactivity Bov, Hu, Ms
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Purified human platelet TSP
Application Immunofluorescence. Immunoprecipitation (10 µg/mg protein lysate; Native and denatured using Protein A). Western Blotting (5-10 µg/ml 2 Hrs. RT Recommended positive control: HUVEC Cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 1 mg/ml
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Thrombospondin represents about 25% of the protein content of platelet a-granules and is secreted at sites of platelet activation and aggregation suggesting that the protein has an important role in hemostasis. Given the involvement of TSP in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells, it is likely that TSP secreted at sites of wounding or clotting plays several important roles in this provisional matrix facilitating the healing process. The mature TSP peptide chain consists of 1152 amino acids and displays an apparent molecular weight of about 170 to 180kD on reducing SDS gels. The trimer of identical subunits of TSP is formed by interchain disulfides. Reacts with Human, Cow, and Mouse. Others not tested. Cellular Localization: Secrotory granules, golgi complex,ER, fibrillar extracellular matrix surrounding the cells.

Mol. Wt. of Antigen: ~ 450kD (Non rreduced) or 170-180kD (reduced)

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