discontinued

DM3213 Thymidylate Synthase antibody

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0.1 mg / €270.00

Quick Overview

Mouse anti Human Thymidylate Synthase TS106

DM3213

Product Description for Thymidylate Synthase

Mouse anti Human Thymidylate Synthase TS106.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for Thymidylate Synthase

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, IP, WB
Clonality Monoclonal
Clone TS106
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Recombinant human TS enzyme
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA (For coating, order Ab without BA). Flow Cytometry. Immunofluorescence. Immunoprecipitation (Native verified): Use Protein G; Ab 2 micrograms/mg protein lysate. Western Blotting: Ab 1-2 micrograms/ml for 2hrs at RT. Immunohistology (formalin/paraffin): Use Ab at 5-10 micrograms/ml for 30 min at RT) -(1:40) Staining of formalinfixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0. Recommended positive control: 5-FU-resistant cell cancer lines or colon carcinoma. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Johnston PG, et. al. Cancer Res, 1991, 51(24):6668-76.
  2. Johnston PG, et. al. Cancer Res, 1992, 52(16):4306-12.
  3. Johnston PG, et. al. Biochem Pharmac, 1993, 45:2483-6.
  4. Johnston PG, et. al. J Clin Oncol, 1994, 12(12):2640-7.
  5. Johnston PG, et. al. Cancer Res, 1995, 55(7):1407-12.
  6. Johnston PG, et. al. J Natl Cancer Inst, 1997, 89(4):308-13.
  7. Pestalozzi BC, et. al. J Clin On
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Purified
Specificity
Specificity:
Thymidylate Synthase (TS) 1 (EC 2.1.1.45) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head, neck, and breast carcinomas. Cellular Localization Cytoplasmic, Nuclear.

Mol. Wt. of Antigen: 36kD

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