NB600-1141 Thymine Dimer antibody

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Mouse anti Chicken Thymine Dimer H3


Product Description for Thymine Dimer

Mouse anti Chicken Thymine Dimer H3.
Presentation: Purified
Product is tested for Dot blot, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Southern blot.

Properties for Thymine Dimer

Product Category Primary Antibodies
Quantity 25 µl
Presentation Purified
Reactivity Chk
Applications Dot, E, ICC/IF, SB
Clonality Monoclonal
Clone H3
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Chemical / Small Molecule.
Isotype control AM03095PU-N
Application ELISA, Immunocytochemistry, May be used for ELISA, ICC, and Southern Blotting., ELISA, Immunocytochemistry,
Background Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection, is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separa-tion by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. The method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal anti-bodies reacting specifically with thymine dimer, facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.
Concentration 2 mg/ml
General Readings General / background references:Marvik OJ et al. Photoimmunodetection: a nonradioactive labeling and detection method for DNA. Biotechniques 23:892-6 (1997). PubMed PMID: 9383556
Roza L et al. Detection of cyclobutane thymine dimers in DNA of human cells with monoclonal antibodies raised against a thymine dimer-containing tetranucleotide. Photochem Photobiol 48:627-33 (1988). PubMed PMID: 3241835
Storage Store at -20C. Avoid freeze-thaw cycles.
Protein G purified
Buffer System:
15mM Sodium Azide
Protein G purified
Monoclonal Anti-Thymine Dimer reacts specifically with the (5?-6?) cyclobutane type of homothymine or thyminecytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides (a tail of minimum 10-20 thymine residues is required for efficient labeling of oligonucleotide probes). The product enables a sensitive and non-radioactive method for labeling, detection, and quantification of DNA fragments using ELISA, competitive ELISA, immunocytochemistry (laser-scan microscopy) and Southern immunoblotting.
Not species specific.

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