AF2310 TIMP1 antibody

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0.1 mg / €150.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Human TIMP1 7-6C1

AF2310

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Product Description for TIMP1

Mouse anti Bovine, Human TIMP1 7-6C1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for TIMP1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CLGI, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, TIMP, TIMP-1, Tissue inhibitor of metalloproteinases
Presentation Purified
Reactivity Bov, Hu
Applications E, P, WB
Clonality Monoclonal
Clone 7-6C1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01033
GeneID:
7076
Application ELISA (See Kodama S. et al. 1990). 
Western blotting: 2 µg/ml (See also Romanic A. et al. 1998)
Immunohistochemistry on Paraffin Sections: 2-25 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity.
Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B).
Concentration 0.5 mg/ml
General Readings
  1. Kodama S, Kishi J, Obata K, Iwata K, Hayakawa T. Monoclonal antibodies to bovine collagenase inhibitor. Coll Relat Res. 1987 Oct;7(5):341-50. PubMed PMID: 3319376.
  2. Kodama S, Iwata K, Iwata H, Yamashita K, Hayakawa T. Rapid one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases. An application for rheumatoid arthritis serum and plasma. J Immunol Methods. 1990 Feb 20;127(1):103-8. PubMed PMID: 2181021.
  3. Agapova OA, Ricard CS, Salvador-Silva M, Hernandez MR. Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human optic nerve head astrocytes. Glia. 2001 Mar 1;33(3):205-16. PubMed PMID: 11241738.
  4. Romanic AM, White RF, Arleth AJ, Ohlstein EH, Barone FC. Matrix metalloproteinase expression increases after cerebral focal ischemia in rats: inhibition of matrix metalloproteinase-9 reduces infarct size. Stroke. 1998 May;29(5):1020-30. PubMed PMID: 9596253.
  5. Zhao WQ, Li H, Yamashita K, Guo XK, Hoshino T, Yoshida S, et al. Cell cycle-associated accumulation of tissue inhibitor of metalloproteinases-1 (TIMP-1) in the nuclei of human gingival fibroblasts. J Cell Sci. 1998 May;111 ( Pt 9):1147-53. PubMed PMID: 9547291.
  6. Fukuda, Y. et al., Gastroenterology. Jpn., 26, 37-41, 1991.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Preservatives:
None
Stabilizers:
0.5% Protease-free BSA
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This monoclonal 7-6C1 antibody specifically reacts with Bovine TIMP-1 and cross-reacts with Human and Rat TIMP-1, but not with Mouse TIMP-1. 
By western blot the antibody recognizes a glycoprotein of ~29 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).
Species:
Human and Bovine.
Gene ID 7076
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for TIMP1 (16 products)

Catalog No. Species Pres. Purity   Source  

TIMP1

TIMP1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

TIMP1

TIMP1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

TIMP1

TIMP1 Human > 90 %
Preparation: .
Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE.
HEK293 cells

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

TIMP1

TIMP1 Human > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
10 µg / €230.00
  Acris Antibodies GmbH

TIMP1

TIMP1 Human > 95 % pure by SDS-PAGE gel and HPLC analyses. E. coli
2 µg / €140.00
  Acris Antibodies GmbH

TIMP1 (24-207, His-tag)

TIMP1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

TIMP1 (24-207, His-tag)

TIMP1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

TIMP1 (24-207, His-tag)

TIMP1 Human Purified > 90 % by SDS - PAGE
0.25 mg / €940.00
  Acris Antibodies GmbH

TIMP1 (24-207, His-tag)

TIMP1 Human Purified > 90 % by SDS - PAGE
50 µg / €300.00
  Acris Antibodies GmbH

TIMP1

TIMP1 Human > 95 % Prepared by affinity chromatography WI-38 cells
5 µg / €270.00
  Acris Antibodies GmbH

TIMP1

TIMP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

TIMP1

TIMP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

TIMP1

TIMP1 Human
10 µg / €290.00
  Abnova Taiwan Corp.

TIMP1 (1-207)

Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
10 µg / €160.00
  Protein Alternatives

TIMP1 (1-207)

Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
25 µg / €230.00
  Protein Alternatives

TIMP1 (1-207)

Human TIMP1 (Metalloproteinase inhibitor 1) recombinant protein Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunoblotting with anti-MBP (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry E. coli
50 µg / €350.00
  Protein Alternatives

Positive controls for TIMP1 (1 products)

Catalog No. Species Pres. Purity   Source  

TIMP1 Lysate

Western Blot: TIMP1 Lysate [NBL1-16924] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for TIMP1.
0.1 mg / €400.00
  Novus Biologicals Inc.
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