NB100-78201 TNF- alpha antibody

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Rat anti Mouse TNF- alpha MP6-XT22


Product Description for TNF- alpha

Rat anti Mouse TNF- alpha MP6-XT22.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for TNF- alpha

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms DIF, MCF
Presentation Aff - Purified
Reactivity Ms
Applications C, F, ICC/IF, WB
Clonality Monoclonal
Clone MP6-XT22
Host Rat
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

E. coli-expressed, recombinant mouse TNF- alpha
Application Immunocytochemistry, flow cytometry / FACS analysis, immunohistochemistry, Western Blot
Background TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomeric mouse TNF- alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF- alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF- alpha has also been described. TNF- alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF- alpha.
General Readings
  1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Beutler, B., et al., 1988. Annu. Rev. Biochem. 57:505. 3. Beutler, B., et al., 1989. Annu. Rev. Immunol. 7:625.
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Immunogen affinity purified
Aff - Purified

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