AM05278PU-N TP53I3 antibody

See related secondary antibodies

Search for all "TP53I3"

0.1 mg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human TP53I3 10A2

Product Description for TP53I3

Mouse anti Human TP53I3 10A2.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for TP53I3

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms PIG3, Quinone oxidoreductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein
Presentation Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone 10A2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q53FA7
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant Human PIG-3 protein.
GeneID:
9540
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot (5-10 µg/ml).
Positive Control: Jurkat cell lysate.
Background P53 Inducible Gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Marusyk A, Wheeler LJ, Mathews CK, DeGregori J. p53 mediates senescence-like arrest induced by chronic replicational stress. Mol Cell Biol. 2007 Aug;27(15):5336-51. Epub 2007 May 21. PubMed PMID: 17515610. (Free PMC Article available, 10 images available)
General Readings
  1. Flatt PM, Polyak K, Tang LJ, Scatena CD, Westfall MD, Rubinstein LA, et al. p53-dependent expression of PIG3 during proliferation, genotoxic stress, and reversible growth arrest. Cancer Lett. 2000 Aug 1;156(1):63-72. PubMed PMID: 10840161.
  2. Venot C, Maratrat M, Dureuil C, Conseiller E, Bracco L, Debussche L. The requirement for the p53 proline-rich functional domain for mediation of apoptosis is correlated with specific PIG3 gene transactivation and with transcriptional repression. EMBO J. 1998 Aug 17;17(16):4668-79. PubMed PMID: 9707426. (Free PMC Article available)
  3. El-Deiry, W.S., Regulation of p53 downstream genes. Semin. Cancer Biol. 1998, 8, 345-357.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS
Preservatives:
0.08% Sodium Azide
Purity:
Purified
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
Recognizes PIG3 (p53 inducible gene 3).
Species:
Human.
Gene ID 9540

Accessory Products

Proteins and/or Positive Controls

Proteins for TP53I3 (7 products)

Catalog No. Species Pres. Purity   Source  

TP53I3 (transcript variant 1)

TP53I3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

TP53I3 (transcript variant 2)

TP53I3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

TP53I3 (1-332, His-tag)

TP53I3 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  OriGene Technologies GmbH

TP53I3 (1-332, His-tag)

TP53I3 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  OriGene Technologies GmbH

TP53I3

TP53I3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TP53I3

TP53I3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TP53I3

SDS-Page: PIG3 Protein [NBP1-44458] - TP53I3, 37.6 kDa (352aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Human Purified
  Novus Biologicals Inc.

Positive controls for TP53I3 (6 products)

Catalog No. Species Pres. Purity   Source  

PIG3 Lysate

Western Blot: PIG3 Lysate [NBL1-17206] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for TP53I3
  Novus Biologicals Inc.

TP53I3 Lysate(Denatured)

TP53I3 Lysate(Denatured)
  Abnova Taiwan Corp.

TP53I3 Lysate(Denatured)

TP53I3 Lysate(Denatured)
  Abnova Taiwan Corp.

TP53I3 overexpression lysate

TP53I3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

TP53I3 overexpression lysate

TP53I3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

TP53I3 overexpression lysate

TP53I3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn