NB100-78474 TPX2 antibody

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Mouse anti Human TPX2 18D5

Product Description for TPX2

Mouse anti Human TPX2 18D5.
Presentation: Biotin
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for TPX2

Product Category Primary Antibodies
Quantity 0.25 mg
Synonyms DIL2
Presentation Biotin
Reactivity Hu
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone 18D5
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Amino Acid: 1-220 of human TPX2
Isotype control SM10B (for use in human samples)
Application immunofluorescence, immunohistochemistry/immunofluorescence, Western Blot
Background The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, alpha-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.
General Readings
  1. Gruss, O. J., et al., 2002. Nature Cell Biol. 4:871.2. Heidebrecht, H. J., et al., 1997. Blood 90:226.3. Kufer, T.A., et al., 2002. J. Cell. Biol. 158:617.
Storage Store at 4C. Do not freeze. Do not freeze.
Immunogen affinity purified
Buffer System:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0
Immunogen affinity purified

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