AP21445SU-N Transferrin (TF) antibody

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1 ml / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Duck Transferrin (TF)

AP21445SU-N

Product Description for Transferrin (TF)

Goat anti Duck Transferrin (TF).
Presentation: Serum
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Transferrin (TF)

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Beta-1 metal-binding globulin, Ovotransferrin
Presentation Serum
Reactivity Duck
Applications C, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P56410
Immunogen:
Purified transferrin isolated from pooled duck serum.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Application This antibody can be used in precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion to identify the presence Transferrin in duck serum or other body fluids and to determine its concentration.
This antiserum has not been tested in more sensitive assays as Nephelometry, ELISA or use in Immunohistology, but this does not exclude such use if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/32 when tested against pooled normal Duck serum in agar-block Immunodiffusion titration.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
State:
Lyophilized, Delipidated, Heat inactivated, Stable Whole Antiserum without preservatives.
Serum
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is restricted to Transferrin as tested in Immunoelectrophoresis and Radial Immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified Transferrin.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction has been observed with chicken and pigeon.
Species:
Duck.

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