BP866 Transforming Growth Factor beta 1 (TGF beta 1) antibody

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Chicken anti Human, Mouse, Porcine Transforming Growth Factor beta 1 (TGF beta 1)


Product Description for Transforming Growth Factor beta 1 (TGF beta 1)

Chicken anti Human, Mouse, Porcine Transforming Growth Factor beta 1 (TGF beta 1).
Presentation: Azide Free
Product is tested for Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.

Properties for Transforming Growth Factor beta 1 (TGF beta 1)

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Azide Free
Reactivity Hu, Ms, Por
Applications E, FN, WB
Clonality Polyclonal
Host Chicken
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Application ELISA (1/500-1/1,500). Western blot (1/500-1/1,500). Neutralization (Neutralises porcine and human TGF-b1 and TGF-b1.2. Reacts with murine TGF-b1. Has a very low affinity for TGF-b2. Does not react with FGF, PDGF, TNFa or b or IL 1, 2, 3, 4, 6 or 7. ND50: Approximately 0.2-0.6 µg/ml in the presence of 0.25 ng/ml of rhTGF-b1, using TGF-b responsive HT-2 cells). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Storage Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Western blotting protocol Blotting:
1. Following electrophoresis, align the gel on a PVDF membrane previously wetted in 100% methanol and transfer buffer.
2. Sandwich the gel and PVDF membrane between two sheets of 3 mm paper and mount in cassettes (see manufacturer's instructions).
3. Blot for 2 hours at 80 volts (constant voltage) with cooling in a Bio-Rad mini Trans- Blot or similar transfer chamber.
4. Store the membrane dry at 4şC overnight.
1. The following day, wet the membrane with 100% methanol for 5 seconds.
2. Rinse in TBS then block in TBS containing 1% EIA grade BSA and 0.05% Tween 20 for 2 hours at room temperature.
1. Dilute the stock solution of anti-TGF beta antibody in blocking buffer to a dilution of 1/500 - 1/1000.
2.Incubate the blocked nitrocellulose with the diluted antibody for 2 hours at room temperature. (To minimise the diluted antibody required for this incubation place the nitrocellulose in a heat seal bag slightly larger than the nitrocellulose, add the diluted antibody, seal the bag and then incubate on a rocker. Under these conditions 10 ml of diluted antibody should be ample for the incubation of a whole gel. Smaller amounts should be sufficient if only a few gel lanes were blotted. The diluted antibody can be re-used at least five times within one month when stored at 2-4şC and with the addition of 0.05% azide.)
3. Wash the nitrocellulose X3 in TBS containing 0.05% Tween 20 for 5 minutes in 100 ml for each wash.
4. Add specific anti-IgY enzyme or biotin labelled antibody at an appropriate dilution. Incubate at room temperature for 1 hour. Wash as in step 3.
5. Use appropriate biotin detection reagents.
6. Un
Azide Free
Reactive with porcine TGF-β.

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